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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2005;25:334.)
© 2005 American Heart Association, Inc.

Vascular Biology

Superoxide Dismutase Inhibits the Expression of Vascular Cell Adhesion Molecule-1 and Intracellular Cell Adhesion Molecule-1 Induced by Tumor Necrosis Factor- in Human Endothelial Cells Through the JNK/p38 Pathways

Shing-Jong Lin; Song-Kun Shyue; Ya-Yun Hung; Yung-Hsiang Chen; Hung-Hai Ku; Jaw-Wen Chen; Ka-Bik Tam; Yuh-Lien Chen

From the Institute of Clinical Medicine (S.-J.L., Y.-H.C., J.-W.C.), Cardiovascular Research Center (S.-J.L.), and the Institute of Anatomy and Cell Biology (Y.-Y.H., H.-H.K., Y.-L.C.), National Yang-Ming University; the Institute of Biomedical Science (S.-K.S., K.-B.T.), Academia Sinica; and the Division of Cardiology (S.-J.L., J.-W.C.), Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China.

Correspondence to Dr Yuh-Lien Chen, Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, No. 1, Section 1, Ren-Ai Rd, Taipei, 100, Taiwan. E-mail ylchen{at}ha.mc.ntu.edu.tw

Objective— Expression of adhesion molecules on endothelial cells and subsequent leukocyte recruitment are critical early events in the development of atherosclerosis. We tried to study possible effects of Cu/Zn superoxide dismutase (SOD) on adhesion molecule expression and its underlying mechanism in the prevention and treatment of cardiovascular disorders.

Methods and Results— Human aortic endothelial cells (HAECs) were transfected with adenovirus carrying the human SOD gene (AdSOD) to investigate whether SOD expression in HAECs attenuated tumor necrosis factor (TNF)-–induced reactive oxygen species production and adhesion molecule expression and to define the mechanisms involved. SOD expression significantly suppressed TNF-–induced expression of vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1 and reduced the binding of the human neutrophils to TNF-–stimulated HAECs. SOD expression suppressed c-JUN N-terminal kinase and p38 phosphorylation. It also attenuated intracellular superoxide anion production and NADPH oxidase activity in TNF-–treated HAECs.

Conclusions— These results provide evidence that SOD expression in endothelial cells attenuates TNF-–induced superoxide anion production and adhesion molecule expression, and that this protective effect is mediated by decreased JNK and p38 phosphorylation and activator protein-1 and nuclear factor B inactivation. These results suggest that SOD has antiinflammatory properties and may play important roles in the prevention of atherosclerosis and inflammatory response.

Superoxide dismutase overexpression in endothelial cells attenuates tumor necrosis factor-–induced superoxide anion production and adhesion molecule expression, and this effect is mediated by decreased JNK and p38 phosphorylation and AP-1 and nuclear factor B inactivation. These results suggest that superoxide dismutase may play an important role in the prevention of atherosclerosis and inflammatory response.

Key Words: superoxide dismutase • atherosclerosis • endothelial cell • adhesion molecule • MAPKs

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