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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2004;24:1549.)
© 2004 American Heart Association, Inc.

Brief Reviews

Heparin-Binding Domains in Vascular Biology

Eva M. Muñoz; Robert J. Linhardt

From the Departments of Chemistry and Chemical Biology (E.M.M., R.J.L.) and Chemical and Biological Engineering (R.J.L.), Department of Biology, Rensselaer Polytechnic Institute, Troy, NY.

Correspondence to Robert J. Linhardt, Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180. E-mail linhar{at}rpi.edu

Heparin is a major anticoagulant with activity mediated primarily through its interaction with antithrombin (AT). Heparan sulfate (HS), structurally related to heparin, binds a wide range of proteins of different functionality, taking part in various physiological and pathological processes. The heparin–AT complex, the most well understood facet of anticoagulation, serves as a prototypical example of the important role of heparin/HS in vascular biology. Extensive studies have identified common structural features in heparin/HS–binding sites of proteins. These include the elucidation of consensus sequences in proteins, patterns of clusters of basic and nonbasic residues, and common spatial arrangements of basic amino acids in the heparin-binding sites. Although these studies have provided valuable information, heparin/HS–binding proteins differ widely in structure. The prediction of heparin/HS–binding proteins from sequence information is not currently possible, and elucidation of protein-binding sites requires the individual study of each glycosaminoglycan–protein complex. Thus, x-ray crystallography and site-directed mutagenesis experiments are among the most powerful tools, providing accurate structural information, facilitating the characterization of heparin–protein complexes.

Heparin and structurally related heparan sulfate bind a large number of proteins, taking part in a wide range of biological processes, particularly ones involved in vascular biology. Heparin-binding domains share certain common structural features, but there is no absolute dependency on specific sequences or protein folds.

Key Words: heparin-binding domains • glycosaminoglycan • basic amino acids • consensus sequence • antithrombin

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