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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2004;24:2333.)
© 2004 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Stem Cell Transplantation Reveals That Absence of Macrophage CD36 Is Protective Against Atherosclerosis

Maria Febbraio; Ella Guy; Roy L. Silverstein

From the Department of Cell Biology (M.F., R.L.S.), Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio; and the Department of Medicine (E.G.), Division of Hematology/Medical Oncology, Weill Medical College of Cornell University, New York, NY.

Correspondence to Maria Febbraio, PhD, Cell Biology, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio. E-mail febbram{at}ccf.org

Objective— CD36 is expressed on multiple cell types and has numerous functions, a subset of which can impact on atherogenesis. In previous work, we demonstrated that CD36 absence was protective against lesion formation. The current objective was to determine whether absence of macrophage CD36 alone was protective.

Methods and Results— Lethal irradiation and stem cell transfer were used to create chimeric mice that did or did not express macrophage CD36 in the context of the Apo E-null model of atherosclerosis. After engraftment, mice were fed a Western diet for 12 weeks. White cell counts, plasma levels of lipoproteins, triacylglycerol, and nonesterified fatty acids were determined, and glucose tolerance tests were preformed. Lesion area was assessed quantitatively after oil red O staining. Mice lacking CD36 in macrophages alone were profoundly protected against atherosclerosis (88.1% reduction of lesion area throughout the aortic tree). Re-introduction of macrophage CD36 resulted in a 2.11-fold increase in lesion area. There were no differences in engraftment, macrophage recruitment, glucose tolerance, weight, and total, low-density lipoprotein, and high-density lipoprotein cholesterol among the groups. Lesions contained similar percent macrophage antigen-positive area.

Conclusion— Protection in this model is primarily caused by loss of CD36 macrophage function.

To determine whether absence of macrophage CD36 alone was protective against atherosclerosis, mice were lethally irradiated and reconstituted with CD36 wild-type or null stem cells in the context of Apo E-null mice lacking CD36 in macrophages alone were profoundly protected against atherosclerosis (88.1% reduction of lesion area).

Key Words: CD36 • atherosclerosis • Apo E-null mice • scavenger receptor • macrophages

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