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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2004;24:2181.)
© 2004 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

High-Density Lipoprotein Subpopulation Profile and Coronary Heart Disease Prevalence in Male Participants of the Framingham Offspring Study

Bela F. Asztalos; L. Adrienne Cupples; Serkalem Demissie; Katalin V. Horvath; Caitlin E. Cox; Marcelo C. Batista; Ernst J. Schaefer

From the Lipid Metabolism Laboratory (B.F.A., K.V.H., C.E.C., M.C.B., E.J.S.), HNRCA at Tufts University, Boston, Mass; and the Department of Biostatistics (L.A.C., S.D.), Boston University, Mass.

Correspondence to Dr Bela F. Asztalos, JM-USDA/HNRC at Tufts University, Lipid Metabolism Laboratory, 711 Washington Street, Boston, MA 02111. E-mail bela.asztalos{at}tufts.edu

Objective— High-density lipoprotein (HDL) is a heterogeneous lipoprotein class and there is no consensus on the value of HDL subspecies in coronary heart disease (CHD) risk assessment. We tested the hypothesis whether specific HDL subpopulations are significantly associated with CHD-prevalence.

Methods and Results— ApoA-I concentrations (mg/dL) in HDL subpopulations were quantitatively determined by native 2d gel electrophoresis, immunoblotting, and image analysis in male participants in the Framingham Offspring Study (FOS). CHD cases (n=169) had higher preß-1 and -3 particle and lower -1, pre-3, and pre-1 particle levels than either all (n=1277) or HDL cholesterol-matched (n=358) controls. -1 and pre-3 levels had an inverse association, whereas -3 and pre-1 particle levels had a positive association with CHD prevalence after adjusting the data for established CHD risk factors. Standardized logit coefficients indicated that -1 HDL was most significantly associated with CHD prevalence. Moreover, each mg/dL increase in -1 particle level decreased odds of CHD by 26% (P<0.0001), whereas each mg/dL increase in HDL cholesterol decreased odds of CHD by 2% in a model including all established CHD risk factors.

Conclusions— Specific HDL subpopulations were positively correlated, whereas others were inversely correlated with CHD prevalence in male subject in the FOS, indicating that the various HDL particles might have different roles in the cause of CHD.

Specific HDL subpopulations were positively correlated whereas others were inversely correlated with coronary heart disease (CHD) prevalence in male subjects in the Framingham Offspring Study, indicating that the various HDL particles might have different roles in the cause of CHD. Moreover, specific HDL particles had stronger association with CHD prevalence than either HDL cholesterol or LDL cholesterol levels.

Key Words: lipoproteins • high-density lipoprotein cholesterol • high-density lipoprotein subpopulations • apolipoprotein A-I • coronary heart disease

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