This Article Full Text Full Text (PDF) All Versions of this Article: 23/9/1653    most recent 01.ATV.0000089328.23279.3Fv1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kujiraoka, T. Articles by Miller, N. E. Search for Related Content PubMed PubMed Citation Articles by Kujiraoka, T. Articles by Miller, N. E. Related Collections Lipids Lipid and lipoprotein metabolism

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2003;23:1653.)
© 2003 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Effects of Intravenous Apolipoprotein A-I/Phosphatidylcholine Discs on LCAT, PLTP, and CETP in Plasma and Peripheral Lymph in Humans

Takeshi Kujiraoka; M. Nazeem Nanjee; Tomoichiro Oka; Mayumi Ito; Makoto Nagano; C. Justin Cooke; Sadao Takahashi; Waldemar L. Olszewski; Jinny S. Wong; Irina P. Stepanova; Robert L. Hamilton; Tohru Egashira; Hiroaki Hattori; Norman E. Miller

From the Department of Advanced Medical Technology and Development (T.K., T.O., M.I., M.N., T.E., H.H.), BML Inc, Saitama, Japan; Department of Cardiovascular Biochemistry (M.N.N., C.J.C., I.P.S., N.E.M.), St Bartholomew’s and the Royal London School of Medicine and Dentistry, London, UK; Third Department of Internal Medicine (S.T.), Fukui Medical University, Fukui, Japan; Medical Research Center (W.L.O.), Polish Academy of Sciences, Warsaw, Poland; Cardiovascular Research Institute and Department of Anatomy (J.S.W., R.L.H.), University of California, San Francisco, Calif.

Correspondence to Hiroaki Hattori, PhD, Department of Advanced Medical Technology and Development, BML, Inc, 1361-1 Matoba, Kawagoe, Saitama 350-1101, Japan. E-mail hhiro{at}bml.co.jp

Objective— We have previously shown that intravenous apolipoprotein A-I/phosphatidylcholine (apoA-I/PC) discs increase plasma pre-ß HDL concentration and stimulate reverse cholesterol transport (RCT) in humans. We have now investigated the associated changes in the following 3 HDL components that play key roles in RCT: lecithin:cholesterol acyltransferase (LCAT), cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP).

Methods and Results— apoA-I/PC discs (40 mg/kg over 4 hours) were infused into 8 healthy men. Samples of blood and prenodal peripheral lymph were collected for 24 to 48 hours. At 12 hours, plasma LCAT concentration had increased by 0.40±0.90 mg/L (+7.8%; mean±SD; P<0.05), plasma cholesterol esterification rate by 29.0±9.0 nmol/mL per h (+69.5%; P<0.01), plasma CETP concentration by 0.5±0.2 mg/L (+29.7%; P<0.01), and plasma PLTP activity by 1.45±0.67 µmol/mL per h (+23.9%; P<0.01). In contrast, plasma PLTP concentration had decreased by 4.4±2.7 mg/L (-44.8%; P<0.01). The changes in PLTP were accompanied by alterations in the relative proportions of large lipoproteins containing inactive PLTP and small particles containing PLTP of high specific activity. No changes were detected in peripheral lymph.

Conclusions— Nascent HDL secretion may induce changes in PLTP, LCAT, and CETP that promote RCT by catalyzing pre-ß HDL production, cholesterol esterification in HDLs, and cholesteryl ester transfer from HDLs to other lipoproteins.

Key Words: apolipoprotein A-I • phospholipid transfer protein • lecithin • cholesterol acyltransferase • cholesteryl ester transfer protein

This article has been cited by other articles:

				M. C. Cheung and J. J. Albers Active plasma phospholipid transfer protein is associated with apoA-I- but not apoE-containing lipoproteins J. Lipid Res., June 1, 2006; 47(6): 1315 - 1321. [Abstract] [Full Text] [PDF]

				B. Olchawa, B. A. Kingwell, A. Hoang, L. Schneider, O. Miyazaki, P. Nestel, and D. Sviridov Physical Fitness and Reverse Cholesterol Transport Arterioscler. Thromb. Vasc. Biol., June 1, 2004; 24(6): 1087 - 1091. [Abstract] [Full Text] [PDF]