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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2003;23:608.)
© 2003 American Heart Association, Inc.

Vascular Biology

Changes in Perlecan Expression During Vascular Injury

Role in the Inhibition of Smooth Muscle Cell Proliferation in the Late Lesion

Michael G. Kinsella; Phan-Kiet Tran; Mary C.M. Weiser-Evans; Michael Reidy; Richard A. Majack; Thomas N. Wight

From the Departments of Pathology (M.G.K., P.-K.T., M.R., T.N.W.), University of Washington, and the Hope Heart Institute (M.G.K., T.N.W.), Seattle, Wash, and the Departments of Pediatrics and Cell and Structural Biology (M.C.M.W.-E., R.A.M.), University of Colorado Health Sciences Center, Denver.

Correspondence to Michael G. Kinsella, Hope Heart Institute, 1124 Columbia St, Ste 783, Seattle WA 98104. E-mail mkinsella{at}hopeheart.org

Objective— Vascular smooth muscle cells (SMCs), activated by growth factors after arterial injury, migrate and proliferate to expand the intima of the blood vessel. During intimal expansion, proliferation is suppressed and an increasingly large proportion of the neointimal mass is composed of newly synthesized extracellular matrix (ECM). We sough to determine whether the ECM heparan sulfate proteoglycan (HSPG) perlecan, which inhibits SMC proliferation in vitro, also accumulates and limits SMC proliferation during neointimal expansion.

Methods and Results— Perlecan expression and accumulation were analyzed by immunohistochemistry and in situ hybridization during neointima formation after balloon catheter injury to the rat carotid artery. Perlecan expression was low in uninjured vessels and up to 7 days after injury, during maximal SMC proliferation. By 14 days after injury, perlecan was dramatically increased, and immunostaining remained heavy throughout the advanced lesion, 35 to 42 days after injury. Finally, explants of intimal tissue from 35- to 42-day neointimal lesions were digested with glycosaminoglycanases to determine whether endogenous HSPGs inhibit intimal SMC proliferation. SMCs within HS-depleted, but not chondroitinase ABC–treated or mock-incubated, explants were found to proliferate in response to platelet-derived growth factor BB.

Conclusions— HSPGs, such as perlecan, may inhibit the proliferative response of SMCs after vascular injury.

Key Words: heparan sulfate • perlecan • neointimal hyperplasia • vascular smooth muscle • proliferation

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