Published online before print August 14, 2003, doi: 10.1161/01.ATV.0000090673.96120.67
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© 2003 American Heart Association, Inc.
Thrombosis |
Chondroitin Sulfate Anticoagulant Activity Is Linked to Water Transfer
Relevance to Proteoglycan Structure in Atherosclerosis
From the Department of Internal Medicine (M.M.) and Department of Pathology (W.D.W.), Wake Forest University School of Medicine, Winston-Salem, NC.
Correspondence to Maria McGee, MD, Department of Medicine, Rheumatology Section, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157. E-mail mmcgee{at}wfubmc.edu
Objective— Changes in chondroitin sulfate (CS) proteoglycan (PG) during atherosclerosis are associated with chronic inflammatory changes and increased incidence of thrombosis. To explore how glycosaminoglycan changes could influence the thrombogenicity of atherosclerotic lesions, water-transfer reactions were examined during activation of antithrombin by CS.
Methods and Results— Advanced type IV atherosclerotic lesions prone to thrombosis contained CSPG (versican) with undersulfated CS relative to CS of the adjacent healthy aorta. Approximately 11% of the CS disaccharide in versican from healthy arteries was oversulfated, but this proportion decreased markedly to 3% in atherosclerotic lesions. Oversulfated CS functionally bound antithrombin with a dissociation constant of 3.3±1.9 µmol/L. Measured by osmotic stress (OS) techniques with an 
26-Å probe, the reaction was linked to transfer of 2500 mol water per mole of coagulation factor Xa inhibited. Under OS, the anticoagulant efficiency of CS was 1.3 (µmol/L)-1 · s-1, 5- and 15-fold higher than heparan sulfate efficiency measured under OS and standard conditions, respectively.
Conclusions— Decreased sulfation of high molecular weight CSPG in the advancing atherosclerotic lesions may predispose the lesions to thrombosis by disrupting osmotic regulation, limiting avidity for antithrombin and decreasing activation efficiency.
Key Words: atherosclerosis • osmotic stress • antithrombin • chondroitin sulfate proteoglycan • coagulation
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