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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22:1489.)
© 2002 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Microsomal Triglyceride Transfer Protein Gene Expression and Triglyceride Accumulation in Hypoxic Human Hearts

Lars B. Nielsen; Mario Perko; Henrik Arendrup; Claus B. Andersen

From the Department of Clinical Biochemistry (L.B.N.), Department of Thoracic Surgery (M.P., H.A.), and Department of Pathology (C.B.A.), Rigshospitalet, University of Copenhagen, Denmark.

Correspondence to Lars B. Nielsen, MD, PhD, DMSc, Department of Clinical Biochemistry KB3011, Rigshospitalet, University of Copenhagen, Blegdamsvej 9 DK-2100 Copenhagen, Denmark. E-Mail larsbo{at}rh.dk

Objectives— Cardiac myocytes secrete apolipoprotein (apo)B-containing lipoproteins. Their function may be the removal of triglycerides when ß-oxidation of fatty acids is decreased, eg, during hypoxia. To test this hypothesis, we examined heart biopsies from patients undergoing coronary artery bypass graft (CABG, n=13) or valve replacement (n=6) surgery.

Methods and Results— Ventricular microsomal triglyceride transfer protein (P=0.02) and apoB (P=0.04) mRNA levels were both 2-fold higher in CABG compared with valve replacement patients. In CABG patients, ventricular microsomal triglyceride transfer protein mRNA levels were negatively associated with the triglyceride content in ventricular myocytes (r=-0.70; P=0.02) and with mRNA levels of sterol regulatory element binding protein-1 (r=-0.74; P=0.004).

Conclusions— The results are compatible with the notion that cardiac lipoprotein production is increased in hypoxic human ventricle, possibly as a result of decreased sterol regulatory element binding protein-1 expression. This might attenuate accumulation of triglycerides in cardiac myocytes.

Key Words: hypoxia • lipids • lipoproteins • myocytes

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