This Article Full Text Full Text (PDF) All Versions of this Article: 22/4/644    most recent 01.ATV.0000012455.62765.BFv1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dimayuga, P. Articles by Nilsson, J. Search for Related Content PubMed PubMed Citation Articles by Dimayuga, P. Articles by Nilsson, J. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CHOLESTEROL Related Collections Cardiovascular imaging agents/Techniques Acute coronary syndromes CV surgery: aortic and vascular disease

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22:644.)
© 2002 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Inhibitory Effect on Arterial Injury-Induced Neointimal Formation by Adoptive B-Cell Transfer in Rag-1 Knockout Mice

Paul Dimayuga; Bojan Cercek; Sumito Oguchi; Gunilla Nordin Fredrikson; Juliana Yano; Prediman K. Shah; Stefan Jovinge; Jan Nilsson

From Experimental Cardiovascular Research (P.D., G.N.F., S.J., J.N.), Department of Medicine, Lund University, University Hospital MAS, Malmö, Sweden; the Atherosclerosis Research Center (P.D., B.C., S.O., J.Y., P.K.S.), Burns and Allen Research Institute, Division of Cardiology, Cedars-Sinai Medical Center/UCLA School of Medicine, Los Angeles, Calif; and the Department of Biomedical Laboratory Science, Health, and Society (G.N.F.), Malmö University, Malmö, Sweden.

Correspondence to Paul Dimayuga, PhD, Davis Research Building, Room 1064, Cedars-Sinai Medical Center, 8700 Beverly Blvd, Los Angeles, CA 90048. E-mail DimayugaP{at}cshs.org

We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020±0.0160 [n=8] versus 0.0049±0.0022 [n=8] mm², respectively; P<0.05) and in western-type diet–fed Rag-1 KO mice compared with WT mice (0.0312±0.0174 [n=7] versus 0.0050±0.0028 [n=6] mm², respectively; P<0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed normal chow) reduced neointimal formation compared with the effect in unreconstituted Rag-1 KO mice (0.0076±0.0039 [n=9] versus 0.020±0.0160 [n=8] mm², respectively; P<0.05). Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed a western diet) reduced neointimal formation compared the effect in Rag-1 KO mice (0.0087±0.0037 [n=8] versus 0.0312±0.0174 [n=7] mm², respectively; P<0.05). Injured carotid arteries from reconstituted Rag-1 KO mice had detectable IgM and IgG, indicating viable transfer of B cells. The results suggest that B cells modulate the response to arterial injury.

Key Words: neointima • B cells • arterial injury • Rag-1 knockout mice

This article has been cited by other articles:

				P. C. Dimayuga, F. H. Y. Cesena, K.-Y. Chyu, J. Yano, A. Amorn, M. C. Fishbein, P. K. Shah, and B. Cercek Natural antibodies and complement modulate intimal thickening after arterial injury Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2009; 297(5): R1593 - R1600. [Abstract] [Full Text] [PDF]

				A. Strom, M. Wigren, A. Hultgardh-Nilsson, A. Saxena, M. F. Gomez, S. Cardell, G. N. Fredrikson, and J. Nilsson Involvement of the CD1d Natural Killer T Cell Pathway in Neointima Formation After Vascular Injury Circ. Res., October 12, 2007; 101(8): e83 - e89. [Abstract] [Full Text] [PDF]

				P. C. Dimayuga, H. Li, K.-Y. Chyu, G. N. Fredrikson, J. Nilsson, M. C. Fishbein, P. K. Shah, and B. Cercek T Cell Modulation of Intimal Thickening After Vascular Injury: The Bimodal Role of IFN-{gamma} in Immune Deficiency Arterioscler Thromb Vasc Biol, December 1, 2005; 25(12): 2528 - 2534. [Abstract] [Full Text] [PDF]

				Y. Shoenfeld, R. Wu, L. D. Dearing, and E. Matsuura Are Anti-Oxidized Low-Density Lipoprotein Antibodies Pathogenic or Protective? Circulation, October 26, 2004; 110(17): 2552 - 2558. [Full Text] [PDF]

				E. P. Gurfinkel, R. Leon de la Fuente, O. Mendiz, and B. Mautner Flu vaccination in acute coronary syndromes and planned percutaneous coronary interventions (FLUVACS) Study: One-year follow-up Eur. Heart J., January 1, 2004; 25(1): 25 - 31. [Abstract] [Full Text] [PDF]

				G. N. Fredrikson, I. Soderberg, M. Lindholm, P. Dimayuga, K.-Y. Chyu, P. K. Shah, and J. Nilsson Inhibition of Atherosclerosis in ApoE-Null Mice by Immunization with ApoB-100 Peptide Sequences Arterioscler Thromb Vasc Biol, May 1, 2003; 23(5): 879 - 884. [Abstract] [Full Text] [PDF]

				G. K. Hansson The B Cell: A Good Guy in Vascular Disease? Arterioscler Thromb Vasc Biol, April 1, 2002; 22(4): 523 - 524. [Full Text] [PDF]