This Article Full Text Full Text (PDF) Data Supplement Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by El Hadri, K. Articles by Feve, B. Search for Related Content PubMed PubMed Citation Articles by El Hadri, K. Articles by Feve, B. Related Collections Gene expression Gene regulation Other Vascular biology

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22:89.)
© 2002 American Heart Association, Inc.

Vascular Biology

Semicarbazide-Sensitive Amine Oxidase in Vascular Smooth Muscle Cells

Differentiation-Dependent Expression and Role in Glucose Uptake

Khadija El Hadri; Marthe Moldes; Nathalie Mercier; Marise Andreani; Jacques Pairault; Bruno Feve

From the Centre de Recherches Biomédical des Cordeliers, Université Pierre et Marie Curie, Paris, France.

Correspondence to Dr Khadija El Hadri, Centre de Recherches Biomédical des Cordeliers, Université Pierre et Marie Curie, UMR 7079 CNRS, 15 rue de l’Ecole de Médecine, 75270 Paris Cedex 06, France. E-mail Khadija.El-Hadri{at}bhdc.jussieu.fr

Cultured vascular smooth muscle cells (VSMCs) derived from rat aortic media were used to examine semicarbazide-sensitive amine oxidase (SSAO) expression during their differentiation process. In a defined serum-free medium permissive for in vitro VSMC differentiation, there was a large increase in SSAO mRNA and protein levels and in the related enzyme activity during the course of cell culture. This pattern of expression was concomitant with that of some smooth muscle–specific mRNA markers of differentiation. mRNAs in differentiated cultured VSMCs were comparable to those detected in total aorta and media. Pharmacological properties of SSAO present in VSMCs were similar to enzyme activities previously described in the aortic wall. In this model, we also demonstrated that methylamine, a physiological substrate of SSAO, activated 2-deoxyglucose transport in a time- and dose-dependent manner. This methylamine effect was reproduced by other SSAO substrates and was prevented by the SSAO inhibitor semicarbazide. It was antagonized in the presence of catalase, suggesting that SSAO-activated glucose transport was mediated through H₂O₂ production. In addition, methylamine promoted glucose transporter 1 accumulation at the cell surface. Thus, we demonstrate for the first time the differentiation-dependent expression of SSAO in VSMCs and its role in the regulation of VSMC glucose uptake.

Key Words: semicarbazide-sensitive amine oxidase • smooth muscle cells • differentiation • hydrogen peroxide • glucose transport

This article has been cited by other articles:

				A. M. O'Rourke, E. Y. Wang, A. Miller, E. M. Podar, K. Scheyhing, L. Huang, C. Kessler, H. Gao, H.-T. Ton-Nu, M. T. MacDonald, et al. Anti-Inflammatory Effects of LJP 1586 [Z-3-Fluoro-2-(4-methoxybenzyl)allylamine Hydrochloride], an Amine-Based Inhibitor of Semicarbazide-Sensitive Amine Oxidase Activity J. Pharmacol. Exp. Ther., February 1, 2008; 324(2): 867 - 875. [Abstract] [Full Text] [PDF]

				N. Mercier, M. Osborne-Pellegrin, K. El Hadri, A. Kakou, C. Labat, L. Loufrani, D. Henrion, P. Challande, S. Jalkanen, B. Feve, et al. Carotid arterial stiffness, elastic fibre network and vasoreactivity in semicarbazide-sensitive amine-oxidase null mouse Cardiovasc Res, November 1, 2006; 72(2): 349 - 357. [Abstract] [Full Text] [PDF]

				A. M. Brainard, A. J. Miller, J. R. Martens, and S. K. England Maxi-K channels localize to caveolae in human myometrium: a role for an actin-channel-caveolin complex in the regulation of myometrial smooth muscle K+ current Am J Physiol Cell Physiol, July 1, 2005; 289(1): C49 - C57. [Abstract] [Full Text] [PDF]

				I. Sibon, D. Larrieu, K. el Hadri, N. Mercier, B. Feve, P. Lacolley, C. Labat, D. Daret, J. Bonnet, and J.-M. D. Lamaziere Semicarbazide-sensitive Amine Oxidase in Annulo-aortic Ectasia Disease: Relation to Elastic Lamellae-associated Proteins J. Histochem. Cytochem., November 1, 2004; 52(11): 1459 - 1466. [Abstract] [Full Text] [PDF]

				C. Gokturk, J. Nilsson, J. Nordquist, M. Kristensson, K. Svensson, C. Soderberg, M. Israelson, H. Garpenstrand, M. Sjoquist, L. Oreland, et al. Overexpression of Semicarbazide-Sensitive Amine Oxidase in Smooth Muscle Cells Leads to an Abnormal Structure of the Aortic Elastic Laminas Am. J. Pathol., November 1, 2003; 163(5): 1921 - 1928. [Abstract] [Full Text] [PDF]

				N. Mercier, M. Moldes, K. E. Hadri, and B. Feve Regulation of Semicarbazide-Sensitive Amine Oxidase Expression by Tumor Necrosis Factor-alpha in Adipocytes: Functional Consequences on Glucose Transport J. Pharmacol. Exp. Ther., March 1, 2003; 304(3): 1197 - 1208. [Abstract] [Full Text] [PDF]

				N. Morin, V. Visentin, D. Calise, L. Marti, A. Zorzano, X. Testar, P. Valet, Y. Fischer, and C. Carpene Tyramine Stimulates Glucose Uptake in Insulin-Sensitive Tissues in Vitro and in Vivo via Its Oxidation by Amine Oxidases J. Pharmacol. Exp. Ther., December 1, 2002; 303(3): 1238 - 1247. [Abstract] [Full Text] [PDF]