This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ryan, M. J. Articles by Sigmund, C. D. Search for Related Content PubMed PubMed Citation Articles by Ryan, M. J. Articles by Sigmund, C. D. Related Collections Health policy and outcome research Acute coronary syndromes CV surgery: aortic and vascular disease CV surgery: transplantation, ventricular assistance, cardiomyopathy Genetics of Stroke

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22:42.)
© 2002 American Heart Association, Inc.

Vascular Biology

Endothelial Dysfunction and Blood Pressure Variability in Selected Inbred Mouse Strains

Michael J. Ryan; Sean P. Didion; Deborah R. Davis; Frank M. Faraci; Curt D. Sigmund

From the Department of Internal Medicine (M.J.R., S.P.D., D.R.D., F.M.F., C.D.S.), Department of Pharmacology (F.M.F.), and Department of Physiology and Biophysics (C.D.S.), Cardiovascular Center, University of Iowa, Iowa City.

Correspondence to Curt D. Sigmund, PhD, Professor of Internal Medicine, 2191 Medical Laboratories, University of Iowa, Department of Internal Medicine, Iowa City, IA 52242. E-mail Curt-Sigmund{at}uiowa.edu

The genetic regulation of blood pressure (BP) and endothelial function is likely to be polygenic. Because there is considerable variability in basal BP among inbred mouse strains, the purpose of this study was to determine whether a similar variability in vascular function exists among 7 "normotensive" strains. We tested the hypothesis that compared with mice with higher BPs, mice with lower BPs would have greater aortic endothelial responses to acetylcholine (ACh). Mean BP ranged from 117 to 145 mm Hg among the 7 strains. The responses of aortic rings to ACh, sodium nitroprusside, and papaverine were assessed after submaximal precontraction with prostaglandin F₂. The aortas from all strains relaxed in a concentration-dependent manner to sodium nitroprusside and papaverine, but responses to ACh were markedly impaired in the aortas, but not carotid arteries, from 129P3/J and 129X1/SvJ mice. Aortas from the other strains relaxed normally to ACh. Furthermore, the endothelium-dependent dilators ADP and A23187 caused similar relaxation in 129P3/J, 129X1/SvJ, and C57BL/6J mice. Although the data do not support the initial hypothesis, the impaired aortic response to ACh in the 129 strains is a novel finding and illustrates the potential impact that genetic background can have on vascular responsiveness.

Key Words: aorta • blood pressure • endothelium • inbred mice

This article has been cited by other articles:

				M. L. Modrick, S. P. Didion, C. D. Sigmund, and F. M. Faraci Role of oxidative stress and AT1 receptors in cerebral vascular dysfunction with aging Am J Physiol Heart Circ Physiol, June 1, 2009; 296(6): H1914 - H1919. [Abstract] [Full Text] [PDF]

				K. A. Brown, S. P. Didion, J. J. Andresen, and F. M. Faraci Effect of Aging, MnSOD Deficiency, and Genetic Background on Endothelial Function: Evidence for MnSOD Haploinsufficiency Arterioscler. Thromb. Vasc. Biol., September 1, 2007; 27(9): 1941 - 1946. [Abstract] [Full Text] [PDF]

				M. J. Ryan and G. R. McLemore Jr. Hypertension and impaired vascular function in a female mouse model of systemic lupus erythematosus Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R736 - R742. [Abstract] [Full Text] [PDF]

				M. J. Ryan, G. R. McLemore Jr, and S. T. Hendrix Insulin Resistance and Obesity in a Mouse Model of Systemic Lupus Erythematosus Hypertension, November 1, 2006; 48(5): 988 - 993. [Abstract] [Full Text] [PDF]

				J. J. Andresen, N. I. Shafi, W. Durante, and R. M. Bryan Jr. Effects of carbon monoxide and heme oxygenase inhibitors in cerebral vessels of rats and mice Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H223 - H230. [Abstract] [Full Text] [PDF]

				L. J. Mullins, M. A. Bailey, and J. J. Mullins Hypertension, Kidney, and Transgenics: A Fresh Perspective Physiol Rev, April 1, 2006; 86(2): 709 - 746. [Abstract] [Full Text] [PDF]

				J.-M. Vincent, Y. W. Kwan, S. Lung Chan, C. Perrin-Sarrado, J. Atkinson, and J.-M. Chillon Constrictor and Dilator Effects of Angiotensin II on Cerebral Arterioles Stroke, December 1, 2005; 36(12): 2691 - 2695. [Abstract] [Full Text] [PDF]

				S. P. Didion, D. A. Kinzenbaw, and F. M. Faraci Critical Role for CuZn-Superoxide Dismutase in Preventing Angiotensin II-Induced Endothelial Dysfunction Hypertension, November 1, 2005; 46(5): 1147 - 1153. [Abstract] [Full Text] [PDF]

				S. Veerareddy, C.-L. M. Cooke, P. N. Baker, and S. T. Davidge Gender differences in myogenic tone in superoxide dismutase knockout mouse: animal model of oxidative stress Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H40 - H45. [Abstract] [Full Text] [PDF]

				M. J. Ryan, S. P. Didion, S. Mathur, F. M. Faraci, and C. D. Sigmund Angiotensin II-Induced Vascular Dysfunction Is Mediated by the AT1A Receptor in Mice Hypertension, May 1, 2004; 43(5): 1074 - 1079. [Abstract] [Full Text] [PDF]

				M. J. Ryan, S. P. Didion, S. Mathur, F. M. Faraci, and C. D. Sigmund PPAR{gamma} Agonist Rosiglitazone Improves Vascular Function and Lowers Blood Pressure in Hypertensive Transgenic Mice Hypertension, March 1, 2004; 43(3): 661 - 666. [Abstract] [Full Text] [PDF]

				H. Francois, K. Athirakul, L. Mao, H. Rockman, and T. M. Coffman Role for Thromboxane Receptors in Angiotensin-II-Induced Hypertension Hypertension, February 1, 2004; 43(2): 364 - 369. [Abstract] [Full Text] [PDF]

				K. Kazama, G. Wang, K. Frys, J. Anrather, and C. Iadecola Angiotensin II attenuates functional hyperemia in the mouse somatosensory cortex Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H1890 - H1899. [Abstract] [Full Text] [PDF]

				R. Maestri, A. F. Milia, M. B. Salis, G. Graiani, C. Lagrasta, M. Monica, D. Corradi, C. Emanueli, and P. Madeddu Cardiac Hypertrophy and Microvascular Deficit in Kinin B2 Receptor Knockout Mice Hypertension, May 1, 2003; 41(5): 1151 - 1155. [Abstract] [Full Text] [PDF]

				C.-L. M. Cooke and S. T. Davidge Pregnancy-Induced Alterations of Vascular Function in Mouse Mesenteric and Uterine Arteries Biol Reprod, March 1, 2003; 68(3): 1072 - 1077. [Abstract] [Full Text] [PDF]

				S. P. Didion, M. J. Ryan, L. A. Didion, P. E. Fegan, C. D. Sigmund, and F. M. Faraci Increased Superoxide and Vascular Dysfunction in CuZnSOD-Deficient Mice Circ. Res., November 15, 2002; 91(10): 938 - 944. [Abstract] [Full Text] [PDF]

				S. P. Didion, M. J. Ryan, G. L. Baumbach, C. D. Sigmund, and F. M. Faraci Superoxide contributes to vascular dysfunction in mice that express human renin and angiotensinogen Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1569 - H1576. [Abstract] [Full Text] [PDF]

				S. Morimoto, M. D. Cassell, and C. D. Sigmund Glia- and Neuron-specific Expression of the Renin-Angiotensin System in Brain Alters Blood Pressure, Water Intake, and Salt Preference J. Biol. Chem., August 30, 2002; 277(36): 33235 - 33241. [Abstract] [Full Text] [PDF]