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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2001;21:1421.)
© 2001 American Heart Association, Inc.

Vascular Biology

Overexpression of Allograft Inflammatory Factor-1 Promotes Proliferation of Vascular Smooth Muscle Cells by Cell Cycle Deregulation

Michael V. Autieri; Christopher M. Carbone

From the Departments of Physiology (M.V.A.), and Cardiology (M.V.A., C.M.C.), Cardiovascular Research Group, Temple University School of Medicine, Philadelphia, Pa.

Correspondence to Michael Autieri, PhD, Department of Physiology, Room 810, MRB, Temple University School of Medicine, 3420 N. Broad St, Philadelphia, PA 19140. E-mail mautieri{at}unix.temple.edu

Abstract—— Allograft inflammatory factor-1 (AIF-1) is not present in normal arteries, but its expression is induced in vascular smooth muscle cells (VSMCs) in several models of arterial injury. The proliferation of VSMCs is a major component of neointimal hyperplasia in many arteriopathies, and the purpose of this study was to determine the role of AIF-1 in the growth of VSMCs. Transfection and constitutive expression of AIF-1 in a primary and a rat VSMC line results in enhanced growth of those cells as measured by cell number and is proportional to the amount of AIF-1 expressed. Constitutive expression of AIF-1 results in a shorter cell cycle, as measured by flow cytometry, and aberrant expression of cell cycle proteins, as determined by Western blot. AIF-1 overexpression also permits growth of these cells in serum-reduced media. Collectively, these data suggest that AIF-1 may participate in the progression of vascular proliferative disease on the basis of its ability to regulate the growth of VSMCs.

Key Words: allograft inflammatory factor-1 • vascular smooth muscle cells • vascular injury • proliferation • cell cycle

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