This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kapinsky, M. Articles by Schmitz, G. Search for Related Content PubMed PubMed Citation Articles by Kapinsky, M. Articles by Schmitz, G. Related Collections Pathophysiology Lipid and lipoprotein metabolism Mechanism of atherosclerosis/growth factors

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2001;21:1004.)
© 2001 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Enzymatically Degraded LDL Preferentially Binds to CD14^high CD16⁺ Monocytes and Induces Foam Cell Formation Mediated Only in Part by the Class B Scavenger-Receptor CD36

Michael Kapinsky; Michael Torzewski; Christa Büchler; Chinh Quoc Duong; Gregor Rothe; Gerd Schmitz

From the Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.

Correspondence to Prof Dr Gerd Schmitz, Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany. E-mail gerd.schmitz{at}klinik.uni-regensburg.de

Abstract—Heterogeneity of peripheral blood monocytes is characterized by specific patterns in the membrane expression of Fc -receptor III (FcRIII/CD16) and the lipopolysaccharide receptor (LPS receptor CD14), allowing discrimination of distinct subpopulations. The aim was to analyze the correlation of these phenotypic differences to the early interaction of freshly isolated monocytes with modified lipoproteins by the use of either enzymatically degraded low density lipoprotein (E-LDL), acetylated low density lipoprotein (ac-LDL), oxidized low density lipoprotein (ox-LDL), or native low density lipoprotein. Highest E-LDL binding was observed on CD14^high CD16⁺ monocytes as determined by flow cytometry, suggesting a selective interaction of E-LDL with distinct subpopulations of monocytes. E-LDL induced rapid foam cell formation both in predifferentiated monocyte-derived macrophages and, in contrast to ac-LDL or ox-LDL, also in freshly isolated peripheral blood monocytes. This was accompanied by upregulation of the 2 class B scavenger receptors CLA-1/SR-BI (CD36 and LIMPII Analogous-1/scavenger receptor type B class I) and CD36. Cellular binding and uptake of E-LDL was neither competed by ac-LDL nor the class A scavenger-receptor inhibitor polyinosinic acid but was partially inhibited by an excess of ox-LDL. In predifferentiated monocyte-derived macrophages, an anti-CD36 antibody inhibited cellular binding and uptake of E-LDL by 20%, suggesting that recognition of these hydrolase-modified low density lipoprotein particles is mediated only in part by the class B scavenger receptor CD36.

Key Words: scavenger receptors • CD36 • enzymatically degraded LDL • atherogenesis

This article has been cited by other articles:

				F. K. Swirski, R. Weissleder, and M. J. Pittet Heterogeneous In Vivo Behavior of Monocyte Subsets in Atherosclerosis Arterioscler Thromb Vasc Biol, October 1, 2009; 29(10): 1424 - 1432. [Abstract] [Full Text] [PDF]

				C. A. Gleissner, J. M. Sanders, J. Nadler, and K. Ley Upregulation of Aldose Reductase During Foam Cell Formation as Possible Link Among Diabetes, Hyperlipidemia, and Atherosclerosis Arterioscler Thromb Vasc Biol, June 1, 2008; 28(6): 1137 - 1143. [Abstract] [Full Text] [PDF]

				M. Stec, K. Weglarczyk, J. Baran, E. Zuba, B. Mytar, J. Pryjma, and M. Zembala Expansion and differentiation of CD14+CD16 and CD14++CD16+ human monocyte subsets from cord blood CD34+ hematopoietic progenitors J. Leukoc. Biol., September 1, 2007; 82(3): 594 - 602. [Abstract] [Full Text] [PDF]

				M. Binder, G. Liebisch, T. Langmann, and G. Schmitz Metabolic Profiling of Glycerophospholipid Synthesis in Fibroblasts Loaded with Free Cholesterol and Modified Low Density Lipoproteins J. Biol. Chem., August 4, 2006; 281(31): 21869 - 21877. [Abstract] [Full Text] [PDF]

				R. S. Rosenson-Schloss, E. Chnari, T. A. Brieva, A. Dang, and P. V. Moghe Glutathione Preconditioning Attenuates Ac-LDL-Induced Macrophage Apoptosis via Protein Kinase C-Dependent Ac-LDL Trafficking Experimental Biology and Medicine, January 1, 2005; 230(1): 40 - 48. [Abstract] [Full Text] [PDF]

				M. Torzewski, P. Suriyaphol, K. Paprotka, L. Spath, V. Ochsenhirt, A. Schmitt, S.-R. Han, M. Husmann, V. B. Gerl, S. Bhakdi, et al. Enzymatic Modification of Low-Density Lipoprotein in the Arterial Wall: A New Role for Plasmin and Matrix Metalloproteinases in Atherogenesis Arterioscler Thromb Vasc Biol, November 1, 2004; 24(11): 2130 - 2136. [Abstract] [Full Text] [PDF]