This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nanjee, M. N. Articles by Miller, N. E. Search for Related Content PubMed PubMed Citation Articles by Nanjee, M. N. Articles by Miller, N. E. Related Collections Pathophysiology Lipid and lipoprotein metabolism Other Vascular biology

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2000;20:2148.)
© 2000 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Concentrations of Electrophoretic and Size Subclasses of Apolipoprotein A-I–Containing Particles in Human Peripheral Lymph

M. N. Nanjee; C. J. Cooke; W. L. Olszewski; N. E. Miller

From the Department of Cardiovascular Biochemistry (M.N.N., C.J.C., W.L.O., N.E.M.), St Bartholomew’s and the Royal London School of Medicine and Dentistry, London, UK, and the Department of Surgical Research and Transplantology (W.L.O.), Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.

Correspondence to Prof Norman E. Miller, Department of Cardiovascular Biochemistry, St Bartholomew’s and the Royal London School of Medicine and Dentistry, Charterhouse Square, London EC1M 6BQ, UK. E-mail n.e.miller{at}mds.qmw.ac.uk

Abstract—When cultured cells are exposed to plasma, the initial acceptors of unesterified cholesterol are small lipid-poor apolipoprotein A-I (apoA-I)–containing high density lipoproteins (HDLs) with pre-ß electrophoretic mobility. These are converted by lecithin:cholesterol acyltransferase into larger spheroidal cholesteryl ester–rich HDLs with mobility. To study the determinants of the concentration of small pre-ß HDLs in tissue fluids, we collected prenodal peripheral lymph from 34 fasted normal men. By crossed immunoelectrophoresis, the concentration of pre-ß HDLs in lymph averaged 20% of that in plasma. On multiple regression analysis, pre-ß apoA-I concentration in lymph was directly related to pre-ß apoA-I concentration in plasma and independently to apoA-I concentration in lymph. Similar results were obtained when the same apoA-I–containing particles were quantified by size exclusion chromatography. Lymph pre-ß apoA-I concentration was low in a subject with familial lecithin:cholesterol acyltransferase deficiency, despite a normal plasma pre-ß apoA-I concentration, but was normal in a subject with familial lipoprotein lipase deficiency. These results suggest that the concentration of small pre-ß HDLs in human tissue fluids is determined only in part by the transfer of pre-ß HDLs across capillary endothelium from plasma. Local production, by remodeling of spheroidal HDLs in tissue fluids, may be equally important. Lipolysis of triglyceride-rich lipoproteins by lipoprotein lipase appears to have little effect.

Key Words: apoA-I • lymph • lipoprotein subclasses • LCAT deficiency • LPL deficiency

This article has been cited by other articles:

				C. Cavelier, L. Rohrer, and A. von Eckardstein ATP-Binding Cassette Transporter A1 Modulates Apolipoprotein A-I Transcytosis Through Aortic Endothelial Cells Circ. Res., November 10, 2006; 99(10): 1060 - 1066. [Abstract] [Full Text] [PDF]

				H. Hattori, T. Kujiraoka, T. Egashira, E. Saito, T. Fujioka, S. Takahashi, M. Ito, J. A. Cooper, I. P. Stepanova, M. N. Nanjee, et al. Association of Coronary Heart Disease with Pre-{beta}-HDL Concentrations in Japanese Men Clin. Chem., March 1, 2004; 50(3): 589 - 595. [Abstract] [Full Text] [PDF]

				T. Kujiraoka, M. N. Nanjee, T. Oka, M. Ito, M. Nagano, C. J. Cooke, S. Takahashi, W. L. Olszewski, J. S. Wong, I. P. Stepanova, et al. Effects of Intravenous Apolipoprotein A-I/Phosphatidylcholine Discs on LCAT, PLTP, and CETP in Plasma and Peripheral Lymph in Humans Arterioscler. Thromb. Vasc. Biol., September 1, 2003; 23(9): 1653 - 1659. [Abstract] [Full Text] [PDF]

				C. M. Desrumaux, P. A. Mak, W. A. Boisvert, D. Masson, D. Stupack, M. Jauhiainen, C. Ehnholm, and L. K. Curtiss Phospholipid transfer protein is present in human atherosclerotic lesions and is expressed by macrophages and foam cells J. Lipid Res., August 1, 2003; 44(8): 1453 - 1461. [Abstract] [Full Text] [PDF]

				M. N. Nanjee, C. J. Cooke, R. Garvin, F. Semeria, G. Lewis, W. L. Olszewski, and N. E. Miller Intravenous apoA-I/lecithin discs increase pre-{beta}-HDL concentration in tissue fluid and stimulate reverse cholesterol transport in humans J. Lipid Res., October 1, 2001; 42(10): 1586 - 1593. [Abstract] [Full Text] [PDF]

				A. von Eckardstein, J.-R. Nofer, and G. Assmann High Density Lipoproteins and Arteriosclerosis : Role of Cholesterol Efflux and Reverse Cholesterol Transport Arterioscler. Thromb. Vasc. Biol., January 1, 2001; 21(1): 13 - 27. [Abstract] [Full Text] [PDF]