© 2000 American Heart Association, Inc.
Atherosclerosis and Lipoproteins |
Long-Term Effects of Vitamin E, Vitamin C, and Combined Supplementation on Urinary 7-Hydro-8-Oxo-2'-Deoxyguanosine, Serum Cholesterol Oxidation Products, and Oxidation Resistance of Lipids in Nondepleted Men
From the Research Institute of Public Health (E.P.-S., J.T.S., K.N., J.K., R.S., U.R.), University of Kuopio, Kuopio, Finland; Karolinska Institutet (U.D.), Department of Medical Laboratory Sciences and Technology, Division of Clinical Chemistry, Huddinge University Hospital, Huddinge, Sweden; the German Institute of Human Nutrition (R.B.-F.), Potsdam-Rehbrucke; the Department of Clinical Pharmacology (H.E.P.), Rigshospitalet, University Hospital Copenhagen, Copenhagen, Denmark; and the Institute of Public Health (S.L.), Panum Institute, Copenhagen, Denmark.
Correspondence to Prof Jukka T. Salonen, Research Institute of Public Health, University of Kuopio, PO Box 1627, 70211 Kuopio, Finland. E-mail jukka.salonen{at}uku.fi
Abstract—We studied the
long-term effects of vitamins E and C and their combination on lipid
peroxidation in vivo and in vitro. The Antioxidant Supplementation in
Atherosclerosis Prevention (ASAP) trial is a
double-masked placebo-controlled randomized clinical trial to study the
effects of vitamin C (500 mg of slow release ascorbate per day),
vitamin E (182 mg of RRR-
-tocopherol acetate per day),
and the combination of both antioxidants. Lipid peroxidation
measurements were carried out for 48 male participants at entry and at
12 and 36 months. Compared with placebo, vitamin E and the vitamin
combination increased plasma lipid–standardized
-tocopherol during the first 12 months by 68.2% and
65.2% (P<0.001 for both), respectively, and reduced
serum 7ß-hydroxycholesterol by 50.4%
(P=0.013) and 44.0% (P=0.041),
respectively. The net change of lipid standardized
-tocopherol was 63.8% after 36 months of vitamin E
supplementation and 43.3% for the combination. Vitamin C
supplementation elevated plasma total ascorbate level by 30.1%
(P=0.043) in 12 months and by 91.1%
(P=0.001) in 36 months. Neither vitamin E, vitamin C,
nor the combination influenced the urinary excretion rate of
7-hydro-8-oxo-2'-deoxyguanosine or the antioxidative capacity of
plasma. Vitamin E and the combination of vitamins E and C enhanced the
oxidation resistance of isolated lipoproteins and total serum lipids.
Our data indicate that long-term supplementation of nondepleted men
with a reasonable dose of vitamin E alone or in combination with slow
release vitamin C reduces lipid peroxidation in vitro and in vivo,
whereas a relatively high dose of vitamin C alone does not.
Key Words: antioxidants • lipid peroxidation • oxidation resistance • 7-hydro-8-oxo-2'-deoxyguanosine • oxysterols
This article has been cited by other articles:
 |
|
 |
|
  B. Buijsse, E. J. M. Feskens, L. Kwape, F. J. Kok, and D. Kromhout Both {alpha}- and -Carotene, but Not Tocopherols and Vitamin C, Are Inversely Related to 15-Year Cardiovascular Mortality in Dutch Elderly Men J. Nutr., February 1, 2008; 138(2): 344 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Li, X. Jia, C.-Y. O. Chen, J. B. Blumberg, Y. Song, W. Zhang, X. Zhang, G. Ma, and J. Chen Almond Consumption Reduces Oxidative DNA Damage and Lipid Peroxidation in Male Smokers J. Nutr., December 1, 2007; 137(12): 2717 - 2722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Liu, I-M. Lee, Y. Song, M. Van Denburgh, N. R. Cook, J. E. Manson, and J. E. Buring Vitamin E and Risk of Type 2 Diabetes in the Women's Health Study Randomized Controlled Trial. Diabetes, October 1, 2006; 55(10): 2856 - 2862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N Hathcock, A. Azzi, J. Blumberg, T. Bray, A. Dickinson, B. Frei, I. Jialal, C. S Johnston, F. J Kelly, K. Kraemer, et al. Vitamins E and C are safe across a broad range of intakes Am. J. Clinical Nutrition, April 1, 2005; 81(4): 736 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Micheletta, S. Natoli, M. Misuraca, E. Sbarigia, U. Diczfalusy, and L. Iuliano Vitamin E Supplementation in Patients With Carotid Atherosclerosis: Reversal of Altered Oxidative Stress Status in Plasma but not in Plaque Arterioscler. Thromb. Vasc. Biol., January 1, 2004; 24(1): 136 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Bruunsgaard, H. E. Poulsen, B. K. Pedersen, K. Nyyssonen, J. Kaikkonen, and J. T. Salonen Long-Term Combined Supplementations with {alpha}-Tocopherol and Vitamin C Have No Detectable Anti-Inflammatory Effects in Healthy Men J. Nutr., April 1, 2003; 133(4): 1170 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Salonen, K. Nyyssonen, J. Kaikkonen, E. Porkkala-Sarataho, S. Voutilainen, T. H. Rissanen, T.-P. Tuomainen, V.-P. Valkonen, U. Ristonmaa, H.-M. Lakka, et al. Six-Year Effect of Combined Vitamin C and E Supplementation on Atherosclerotic Progression: The Antioxidant Supplementation in Atherosclerosis Prevention (ASAP) Study Circulation, February 25, 2003; 107(7): 947 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Padayatty, A. Katz, Y. Wang, P. Eck, O. Kwon, J.-H. Lee, S. Chen, C. Corpe, A. Dutta, S. K Dutta, et al. Vitamin C as an Antioxidant: Evaluation of Its Role in Disease Prevention J. Am. Coll. Nutr., February 1, 2003; 22(1): 18 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Linseisen, G. Wolfram, and A. B. Miller Plasma 7{beta}-Hydroxycholesterol as a Possible Predictor of Lung Cancer Risk Cancer Epidemiol. Biomarkers Prev., December 1, 2002; 11(12): 1630 - 1637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ringseis and K. Eder Insufficient Dietary Vitamin E Increases the Concentration of 7{beta}-Hydroxycholesterol in Tissues of Rats Fed Salmon Oil J. Nutr., December 1, 2002; 132(12): 3732 - 3735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Schwenke, L. L. Rudel, M. G. Sorci-Thomas, and M. J. Thomas {alpha}-Tocopherol protects against diet induced atherosclerosis in New Zealand white rabbits J. Lipid Res., November 1, 2002; 43(11): 1927 - 1938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. Jarvik, N. T. Tsai, L. A. McKinstry, R. Wani, V. H. Brophy, R. J. Richter, G. D. Schellenberg, P. J. Heagerty, T. S. Hatsukami, and C. E. Furlong Vitamin C and E Intake Is Associated With Increased Paraoxonase Activity Arterioscler. Thromb. Vasc. Biol., August 1, 2002; 22(8): 1329 - 1333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Bjorkhem and U. Diczfalusy Oxysterols: Friends, Foes, or Just Fellow Passengers? Arterioscler. Thromb. Vasc. Biol., May 1, 2002; 22(5): 734 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H. Ling, L. L. Wang, and J. Ma Supplementation of the Black Rice Outer Layer Fraction to Rabbits Decreases Atherosclerotic Plaque Formation and Increases Antioxidant Status J. Nutr., January 1, 2002; 132(1): 20 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Munzel and J. F. Keaney Jr Are ACE Inhibitors a "Magic Bullet" Against Oxidative Stress? Circulation, September 25, 2001; 104(13): 1571 - 1574. [Full Text] [PDF]
|
|