Arteriosclerosis, Thrombosis, and Vascular Biology. 2000;20:1443-1451
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 2000 American Heart Association, Inc.
Vascular Biology |
Development of Endothelial Cell Lines From Embryonic Stem Cells
A Tool for Studying Genetically Manipulated Endothelial Cells In Vitro
From the Istituto di Ricerche Farmacologiche Mario Negri (G.B., R.S., E.D.), Milan, Italy, and Universita’ degli Studi dell’Insubria (E.D.), Dipartimento di Scienze Cliniche e Biologiche, Facolta’ di Medicina e Chirurgia, Varese, Italy.
Correspondence to Dr Giovanna Balconi, Istituto di Ricerche Farmacologiche "Mario Negri," Via Eritrea 62, 20157 Milan, Italy. E-mail balconi{at}irfmn.mnegri.it
Abstract—Totipotent embryonic stem cells can be induced to differentiate to endothelium in vitro. This may be a useful tool for obtaining cultures of genetically manipulated endothelial cells because embryonic stem cells are relatively easy to transfect and are commonly used for gene inactivation experiments in mice. However, embryonic stem cell–derived endothelial cells could not be easily separated from embryoid bodies and maintained in culture. In this study, we describe the isolation and characterization of immortalized endothelial cell lines obtained from embryonic stem cells differentiated in vitro. The cell lines were analyzed for expression of endothelial cell markers, including growth factor receptors and adhesion molecules, and compared with endothelial cells obtained from the yolk sac, the embryo proper, or the heart microcirculation of the adult. We propose that this approach may be useful for obtaining endothelial cells carrying gene mutations that are lethal at very early stages of development.
Key Words: endothelium • embryonic stem cells • vasculogenesis
This article has been cited by other articles:
 |
|
 |
|
  T. R. Carlson, H. Hu, R. Braren, Y. H. Kim, and R. A. Wang Cell-autonomous requirement for {beta}1 integrin in endothelial cell adhesion, migration and survival during angiogenesis in mice Development, June 15, 2008; 135(12): 2193 - 2202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Aase, M. Ernkvist, L. Ebarasi, L. Jakobsson, A. Majumdar, C. Yi, O. Birot, Y. Ming, A. Kvanta, D. Edholm, et al. Angiomotin regulates endothelial cell migration during embryonic angiogenesis Genes & Dev., August 15, 2007; 21(16): 2055 - 2068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Xiao, L. Zeng, Z. Zhang, Y. Hu, and Q. Xu Stem cell-derived Sca-1+ progenitors differentiate into smooth muscle cells, which is mediated by collagen IV-integrin {alpha}1/beta1/{alpha}v and PDGF receptor pathways Am J Physiol Cell Physiol, January 1, 2007; 292(1): C342 - C352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Pece-Barbara, S. Vera, K. Kathirkamathamby, S. Liebner, G. M. Di Guglielmo, E. Dejana, J. L. Wrana, and M. Letarte Endoglin Null Endothelial Cells Proliferate Faster and Are More Responsive to Transforming Growth Factor {beta}1 with Higher Affinity Receptors and an Activated Alk1 Pathway J. Biol. Chem., July 29, 2005; 280(30): 27800 - 27808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Rossig, C. Urbich, T. Bruhl, E. Dernbach, C. Heeschen, E. Chavakis, K.-i. Sasaki, D. Aicher, F. Diehl, F. Seeger, et al. Histone deacetylase activity is essential for the expression of HoxA9 and for endothelial commitment of progenitor cells J. Exp. Med., June 6, 2005; 201(11): 1825 - 1835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Toporsian, R. Gros, M. G. Kabir, S. Vera, K. Govindaraju, D. H. Eidelman, M. Husain, and M. Letarte A Role for Endoglin in Coupling eNOS Activity and Regulating Vascular Tone Revealed in Hereditary Hemorrhagic Telangiectasia Circ. Res., April 1, 2005; 96(6): 684 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. M. Martinez-Estrada, Y. Munoz-Santos, J. Julve, M. Reina, and S. Vilaro Human adipose tissue as a source of Flk-1+ cells: new method of differentiation and expansion Cardiovasc Res, February 1, 2005; 65(2): 328 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Paik, A. Skoura, S.-S. Chae, A. E. Cowan, D. K. Han, R. L. Proia, and T. Hla Sphingosine 1-phosphate receptor regulation of N-cadherin mediates vascular stabilization Genes & Dev., October 1, 2004; 18(19): 2392 - 2403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Liebner, A. Cattelino, R. Gallini, N. Rudini, M. Iurlaro, S. Piccolo, and E. Dejana {beta}-Catenin is required for endothelial-mesenchymal transformation during heart cushion development in the mouse J. Cell Biol., August 2, 2004; 166(3): 359 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iurlaro, F. Demontis, M. Corada, L. Zanetta, C. Drake, M. Gariboldi, S. Peiro, A. Cano, P. Navarro, A. Cattelino, et al. VE-Cadherin Expression and Clustering Maintain Low Levels of Survivin in Endothelial Cells Am. J. Pathol., July 1, 2004; 165(1): 181 - 189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Spagnuolo, M. Corada, F. Orsenigo, L. Zanetta, U. Deuschle, P. Sandy, C. Schneider, C. J. Drake, F. Breviario, and E. Dejana Gas1 is induced by VE-cadherin and vascular endothelial growth factor and inhibits endothelial cell apoptosis Blood, April 15, 2004; 103(8): 3005 - 3012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Magnusson, C. Rolny, L. Jakobsson, C. Wikner, Y. Wu, D. J. Hicklin, and L. Claesson-Welsh Deregulation of Flk-1/vascular endothelial growth factor receptor-2 in fibroblast growth factor receptor-1-deficient vascular stem cell development J. Cell Sci., March 15, 2004; 117(8): 1513 - 1523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Kaufman, R. L. Lewis, E. T. Hanson, R. Auerbach, J. Plendl, and J. A. Thomson Functional endothelial cells derived from rhesus monkey embryonic stem cells Blood, February 15, 2004; 103(4): 1325 - 1332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cattelino, S. Liebner, R. Gallini, A. Zanetti, G. Balconi, A. Corsi, P. Bianco, H. Wolburg, R. Moore, B. Oreda, et al. The conditional inactivation of the {beta}-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility J. Cell Biol., September 15, 2003; 162(6): 1111 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Lampugnani, A. Zanetti, M. Corada, T. Takahashi, G. Balconi, F. Breviario, F. Orsenigo, A. Cattelino, R. Kemler, T. O. Daniel, et al. Contact inhibition of VEGF-induced proliferation requires vascular endothelial cadherin, {beta}-catenin, and the phosphatase DEP-1/CD148 J. Cell Biol., May 26, 2003; 161(4): 793 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nakul-Aquaronne, J. Bayle, and C. Frelin Coexpression of endothelial markers and CD14 by cytokine mobilized CD34+ cells under angiogenic stimulation Cardiovasc Res, March 1, 2003; 57(3): 816 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Corada, L. Zanetta, F. Orsenigo, F. Breviario, M. G. Lampugnani, S. Bernasconi, F. Liao, D. J. Hicklin, P. Bohlen, and E. Dejana A monoclonal antibody to vascular endothelial-cadherin inhibits tumor angiogenesis without side effects on endothelial permeability Blood, July 18, 2002; 100(3): 905 - 911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Levenberg, J. S. Golub, M. Amit, J. Itskovitz-Eldor, and R. Langer Endothelial cells derived from human embryonic stem cells PNAS, April 2, 2002; 99(7): 4391 - 4396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zanetti, M. G. Lampugnani, G. Balconi, F. Breviario, M. Corada, L. Lanfrancone, and E. Dejana Vascular Endothelial Growth Factor Induces Shc Association With Vascular Endothelial Cadherin: A Potential Feedback Mechanism to Control Vascular Endothelial Growth Factor Receptor-2 Signaling Arterioscler. Thromb. Vasc. Biol., April 1, 2002; 22(4): 617 - 622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Lampugnani, A. Zanetti, F. Breviario, G. Balconi, F. Orsenigo, M. Corada, R. Spagnuolo, M. Betson, V. Braga, and E. Dejana VE-Cadherin Regulates Endothelial Actin Activating Rac and Increasing Membrane Association of Tiam Mol. Biol. Cell, April 1, 2002; 13(4): 1175 - 1189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Condorelli, U. Borello, L. De Angelis, M. Latronico, D. Sirabella, M. Coletta, R. Galli, G. Balconi, A. Follenzi, G. Frati, et al. Cardiomyocytes induce endothelial cells to trans-differentiate into cardiac muscle: Implications for myocardium regeneration PNAS, September 4, 2001; (2001) 191217898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bulotta, R. Barsacchi, D. Rotiroti, N. Borgese, and E. Clementi Activation of the Endothelial Nitric-oxide Synthase by Tumor Necrosis Factor-alpha . A NOVEL FEEDBACK MECHANISM REGULATING CELL DEATH J. Biol. Chem., February 23, 2001; 276(9): 6529 - 6536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Levenberg, J. S. Golub, M. Amit, J. Itskovitz-Eldor, and R. Langer Endothelial cells derived from human embryonic stem cells PNAS, April 2, 2002; 99(7): 4391 - 4396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Condorelli, U. Borello, L. De Angelis, M. Latronico, D. Sirabella, M. Coletta, R. Galli, G. Balconi, A. Follenzi, G. Frati, et al. From the Cover: Cardiomyocytes induce endothelial cells to trans-differentiate into cardiac muscle: Implications for myocardium regeneration PNAS, September 11, 2001; 98(19): 10733 - 10738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zanetti, M. G. Lampugnani, G. Balconi, F. Breviario, M. Corada, L. Lanfrancone, and E. Dejana Vascular Endothelial Growth Factor Induces Shc Association With Vascular Endothelial Cadherin: A Potential Feedback Mechanism to Control Vascular Endothelial Growth Factor Receptor-2 Signaling Arterioscler. Thromb. Vasc. Biol., April 1, 2002; 22(4): 617 - 622. [Abstract] [Full Text] [PDF]
|
|