© 2000 American Heart Association, Inc.
Atherosclerosis and Lipoproteins |
Human ApoA-IV Overexpression in Transgenic Mice Induces cAMP-Stimulated Cholesterol Efflux From J774 Macrophages to Whole Serum
From the Laboratoire de Biochimie Appliquée (N.F., J.-L.P., M.S., N.M.), Faculté des Sciences Pharmaceutiques et Biologiques, Châtenay-Malabry, France; the Laboratoire de Biochimie (N.F., V.A., J.-L.P., N.M.), Hôpital Broussais, Assistance Publique-Hôpitaux de Paris, Paris, France; the Laboratoire Rhône-Poulenc-Rorer (N.D.), Division Gencell, Département d’Athérosclérose, Vitry sur Seine, France; and the MCP Hahnemann School of Medicine (G.H.R.), Biochemistry Department, Philadelphia, Pa.
Correspondence to Dr N. Fournier, Laboratoire de Biochimie, Hôpital Broussais, 96 rue Didot, F-75674 Paris Cedex 14, France. E-mail natalie.fournier{at}brs.ap-hop-paris.fr
Abstract—The role of apolipoprotein A-IV (apoA-IV) in lipoprotein metabolism has not been established. The aim of the present study was to investigate the role of apoA-IV in reverse cholesterol transport by comparing cellular cholesterol efflux to serum or serum fractions from control mice and from mice transgenic for human apoA-IV (HuA-IVTg mice). When Fu5AH hepatoma cells were used, the cholesterol efflux to serum from either control or transgenic mice was similar. When control J774 macrophage cells were used, a comparison of efflux to serum or lipoprotein-deficient serum (LPDS) failed to demonstrate any differences between control and transgenic mice. In contrast, when the J774 cells were pretreated with cAMP, there was a stimulation of efflux to whole serum or LPDS from HuA-IVTg mice. cAMP treatment had no effect on efflux to serum or LPDS from control mice. Pretreatment of the cells with cAMP did not enhance the efflux response to high density lipoprotein isolated from HuA-IVTg mouse serum. Our results suggest that apoA-IV, unassociated with high density lipoprotein particles, is responsible for enhanced cholesterol efflux. This study illustrates the role of lipid-free apolipoproteins in mediating cellular cholesterol efflux with use of a biological fluid and is potentially of physiological relevance, especially in apolipoprotein-rich extravascular fluids.
Key Words: free apolipoprotein A-IV • J774 mouse macrophages • cAMP • cholesterol efflux • transgenic mice
This article has been cited by other articles:
 |
|
 |
|
  M. P. Adorni, F. Zimetti, J. T. Billheimer, N. Wang, D. J. Rader, M. C. Phillips, and G. H. Rothblat The roles of different pathways in the release of cholesterol from macrophages J. Lipid Res., November 1, 2007; 48(11): 2453 - 2462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Westerterp, C. C. van der Hoogt, W. de Haan, E. H. Offerman, G. M. Dallinga-Thie, J. W. Jukema, L. M. Havekes, and P. C.N. Rensen Cholesteryl Ester Transfer Protein Decreases High-Density Lipoprotein and Severely Aggravates Atherosclerosis in APOE*3-Leiden Mice Arterioscler Thromb Vasc Biol, November 1, 2006; 26(11): 2552 - 2559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Spaulding, F. Saijo, R. H. Turnage, J. S. Alexander, T. Y. Aw, and T. J. Kalogeris Apolipoprotein A-IV attenuates oxidant-induced apoptosis in mitotic competent, undifferentiated cells by modulating intracellular glutathione redox balance Am J Physiol Cell Physiol, January 1, 2006; 290(1): C95 - C103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. J. Beulens, A. Sierksma, A. van Tol, N. Fournier, T. van Gent, J-L. Paul, and H. F. J. Hendriks Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1 J. Lipid Res., September 1, 2004; 45(9): 1716 - 1723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liang, X.-C. Jiang, R. Liu, G. Liang, T. P. Beyer, H. Gao, T. P. Ryan, S. Dan Li, P. I. Eacho, and G. Cao Liver X Receptors (LXRs) Regulate Apolipoprotein AIV-Implications of the Antiatherosclerotic Effect of LXR Agonists Mol. Endocrinol., August 1, 2004; 18(8): 2000 - 2010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Favari, M. Lee, L. Calabresi, G. Franceschini, F. Zimetti, F. Bernini, and P. T. Kovanen Depletion of Pre-{beta}-high Density Lipoprotein by Human Chymase Impairs ATP-binding Cassette Transporter A1- but Not Scavenger Receptor Class B Type I-mediated Lipid Efflux to High Density Lipoprotein J. Biol. Chem., March 12, 2004; 279(11): 9930 - 9936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. E. Thorngate, P. G. Yancey, G. Kellner-Weibel, L. L. Rudel, G. H. Rothblat, and D. L. Williams Testing the role of apoA-I, HDL, and cholesterol efflux in the atheroprotective action of low-level apoE expression J. Lipid Res., December 1, 2003; 44(12): 2331 - 2338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Guerin, W. Le Goff, E. Frisdal, S. Schneider, D. Milosavljevic, E. Bruckert, and M. J. Chapman Action of Ciprofibrate in Type IIB Hyperlipoproteinemia: Modulation of the Atherogenic Lipoprotein Phenotype and Stimulation of High-Density Lipoprotein-Mediated Cellular Cholesterol Efflux J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3738 - 3746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ezeh, M. Haiman, H. F. Alber, B. Kunz, B. Paulweber, A. Lingenhel, H.-G. Kraft, F. Weidinger, O. Pachinger, H. Dieplinger, et al. Plasma distribution of apoA-IV in patients with coronary artery disease and healthy controls J. Lipid Res., August 1, 2003; 44(8): 1523 - 1529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Weinberg, R. A. Anderson, V. R. Cook, F. Emmanuel, P. Denefle, A. R. Tall, and A. Steinmetz Interfacial Exclusion Pressure Determines the Ability of Apolipoprotein A-IV Truncation Mutants to Activate Cholesterol Ester Transfer Protein J. Biol. Chem., June 7, 2002; 277(24): 21549 - 21553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fournier, A. Cogny, V. Atger, D. Pastier, D. Goudouneche, A. Nicoletti, N. Moatti, J. Chambaz, J.-L. Paul, and A.-D. Kalopissis Opposite Effects of Plasma From Human Apolipoprotein A-II Transgenic Mice on Cholesterol Efflux From J774 Macrophages and Fu5AH Hepatoma Cells Arterioscler Thromb Vasc Biol, April 1, 2002; 22(4): 638 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Kalogeris and R. G. Painter Adaptation of intestinal production of apolipoprotein A-IV during chronic feeding of lipid Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2001; 280(4): R1155 - R1161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Bortnick, G. H. Rothblat, G. Stoudt, K. L. Hoppe, L. J. Royer, J. McNeish, and O. L. Francone The Correlation of ATP-binding Cassette 1 mRNA Levels with Cholesterol Efflux from Various Cell Lines J. Biol. Chem., September 8, 2000; 275(37): 28634 - 28640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Wang, D. L. Silver, P. Costet, and A. R. Tall Specific Binding of ApoA-I, Enhanced Cholesterol Efflux, and Altered Plasma Membrane Morphology in Cells Expressing ABC1 J. Biol. Chem., October 13, 2000; 275(42): 33053 - 33058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Scott, D. C. McManus, V. Franklin, A. G. McKenzie, T. Neville, D. L. Sparks, and Y. L. Marcel The N-terminal Globular Domain and the First Class A Amphipathic Helix of Apolipoprotein A-I Are Important for Lecithin:Cholesterol Acyltransferase Activation and the Maturation of High Density Lipoprotein in Vivo J. Biol. Chem., December 21, 2001; 276(52): 48716 - 48724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fournier, A. Cogny, V. Atger, D. Pastier, D. Goudouneche, A. Nicoletti, N. Moatti, J. Chambaz, J.-L. Paul, and A.-D. Kalopissis Opposite Effects of Plasma From Human Apolipoprotein A-II Transgenic Mice on Cholesterol Efflux From J774 Macrophages and Fu5AH Hepatoma Cells Arterioscler Thromb Vasc Biol, April 1, 2002; 22(4): 638 - 643. [Abstract] [Full Text] [PDF]
|
|