© 2000 American Heart Association, Inc.
Vascular Biology |
Convergence of Redox-Sensitive and Mitogen-Activated Protein Kinase Signaling Pathways in Tumor Necrosis Factor-
–Mediated Monocyte Chemoattractant Protein-1 Induction in Vascular Smooth Muscle Cells
From the Division of Cardiology, Emory University School of Medicine, Atlanta, Ga.
Correspondence to Kathy K. Griendling, PhD, Emory University, Division of Cardiology, 1639 Pierce Dr, 319 WMB, Atlanta, GA 30322. E-mail kgriend{at}emory.edu
Abstract—Monocyte chemoattractant
protein-1 (MCP-1) is an important component of the inflammatory
response of the vessel wall and has been shown to be regulated by
cytokines, such as tumor necrosis factor-
(TNF-).
However, the precise signaling pathways leading to MCP-1 induction have
not been fully elucidated in vascular smooth muscle cells (VSMCs).
Cytokine signal transduction involves protein kinases as well
as reactive oxygen species (ROS). The relation between these 2 factors
is not clear. In this study, we show that TNF- induces a parallel
phosphorylation of extracellular signal–regulated
kinase 1/2 (ERK1/2) and p38 mitogen-activated protein kinase
(p38MAPK) and increases MCP-1 mRNA expression in cultured VSMCs.
Inhibition of ERK1/2 but not p38MAPK caused a partial attenuation of
MCP-1 induction (43±10% inhibition). Incubation of VSMCs with
multiple antioxidants (diphenylene iodonium, liposomal superoxide
dismutase, catalase, N-acetylcysteine,
dimethylthiourea, and pyrrolidine dithiocarbamate) had
no effect on TNF-–mediated MCP-1 upregulation. However,
simultaneous blockade of the ERK1/2 and ROS pathways by
using PD098059 combined with diphenylene iodonium or
N-acetylcysteine potently enhanced the ability of MAPK
kinase inhibitors to abrogate MCP-1 mRNA expression
(100±2% inhibition). Thus, parallel ROS-dependent and
ERK1/2-dependent pathways converge to regulate TNF-–induced MCP-1
gene expression in VSMCs. These data unmask a complex but organized
integration of ROS and protein kinases that mediates
cytokine-induced vascular inflammatory gene
expression.
Key Words: monocyte chemoattractant protein-1 • smooth muscle cells • reactive oxygen species • cytokines • tyrosine kinase
This article has been cited by other articles:
 |
|
 |
|
  L. Zhang, P. Sivashanmugam, J.-H. Wu, L. Brian, S. T. Exum, N. J. Freedman, and K. Peppel Tumor Necrosis Factor Receptor-2 Signaling Attenuates Vein Graft Neointima Formation by Promoting Endothelial Recovery Arterioscler. Thromb. Vasc. Biol., February 1, 2008; 28(2): 284 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Weiss and W. R. Taylor Deoxycorticosterone Acetate Salt Hypertension in Apolipoprotein E-/- Mice Results in Accelerated Atherosclerosis: The Role of Angiotensin II Hypertension, February 1, 2008; 51(2): 218 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li and H. E. Schellhorn New Developments and Novel Therapeutic Perspectives for Vitamin C J. Nutr., October 1, 2007; 137(10): 2171 - 2184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yang, D. Coriolan, V. Murthy, K. Schultz, D. T. Golenbock, and D. Beasley Proinflammatory phenotype of vascular smooth muscle cells: role of efficient Toll-like receptor 4 signaling Am J Physiol Heart Circ Physiol, September 1, 2005; 289(3): H1069 - H1076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhang, K. Peppel, L. Brian, L. Chien, and N. J. Freedman Vein Graft Neointimal Hyperplasia Is Exacerbated by Tumor Necrosis Factor Receptor-1 Signaling in Graft-Intrinsic Cells Arterioscler. Thromb. Vasc. Biol., December 1, 2004; 24(12): 2277 - 2283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Suzuki, H. Satonaka, H. Nishimatsu, S. Oba, R. Takeda, M. Omata, T. Fujita, R. Nagai, and Y. Hirata Myocyte Enhancer Factor 2 Mediates Vascular Inflammation via the p38-Dependent Pathway Circ. Res., July 9, 2004; 95(1): 42 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-M. Chen, W.-C. Chiang, S.-L. Lin, K.-D. Wu, T.-J. Tsai, and B.-S. Hsieh Dual Regulation of Tumor Necrosis Factor-{alpha}-Induced CCL2/Monocyte Chemoattractant Protein-1 Expression in Vascular Smooth Muscle Cells by Nuclear Factor-{kappa}B and Activator Protein-1: Modulation by Type III Phosphodiesterase Inhibition J. Pharmacol. Exp. Ther., June 1, 2004; 309(3): 978 - 986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Schecter, A. B. Berman, L. Yi, H. Ma, C. M. Daly, K. Soejima, B. J. Rollins, I. F. Charo, and M. B. Taubman MCP-1-dependent signaling in CCR2-/- aortic smooth muscle cells J. Leukoc. Biol., June 1, 2004; 75(6): 1079 - 1085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Rhoden, J. Lawrence, J. J. Godleski, and B. Gonzalez-Flecha N-Acetylcysteine Prevents Lung Inflammation After Short-Term Inhalation Exposure to Concentrated Ambient Particles Toxicol. Sci., June 1, 2004; 79(2): 296 - 303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Satonaka, E. Suzuki, H. Nishimatsu, S. Oba, R. Takeda, A. Goto, M. Omata, T. Fujita, R. Nagai, and Y. Hirata Calcineurin Promotes the Expression of Monocyte Chemoattractant Protein-1 in Vascular Myocytes and Mediates Vascular Inflammation Circ. Res., March 19, 2004; 94(5): 693 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-M. Go, J. J. Gipp, R. T. Mulcahy, and D. P. Jones H2O2-dependent Activation of GCLC-ARE4 Reporter Occurs by Mitogen-activated Protein Kinase Pathways without Oxidation of Cellular Glutathione or Thioredoxin-1 J. Biol. Chem., February 13, 2004; 279(7): 5837 - 5845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Taniyama and K. K. Griendling Reactive Oxygen Species in the Vasculature: Molecular and Cellular Mechanisms Hypertension, December 1, 2003; 42(6): 1075 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yamawaki, S. Lehoux, and B. C. Berk Chronic Physiological Shear Stress Inhibits Tumor Necrosis Factor-Induced Proinflammatory Responses in Rabbit Aorta Perfused Ex Vivo Circulation, September 30, 2003; 108(13): 1619 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Proudfoot, K. D. Croft, I. B. Puddey, and L. J. Beilin Angiotensin II Type 1 Receptor Antagonists Inhibit Basal As Well As Low-Density Lipoprotein and Platelet-Activating Factor-Stimulated Human Monocyte Chemoattractant Protein-1 J. Pharmacol. Exp. Ther., June 1, 2003; 305(3): 846 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kanellis, S. Watanabe, J. H. Li, D. H. Kang, P. Li, T. Nakagawa, A. Wamsley, D. Sheikh-Hamad, H. Y. Lan, L. Feng, et al. Uric Acid Stimulates Monocyte Chemoattractant Protein-1 Production in Vascular Smooth Muscle Cells Via Mitogen-Activated Protein Kinase and Cyclooxygenase-2 Hypertension, June 1, 2003; 41(6): 1287 - 1293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ungvari, A. Csiszar, J. G. Edwards, P. M. Kaminski, M. S. Wolin, G. Kaley, and A. Koller Increased Superoxide Production in Coronary Arteries in Hyperhomocysteinemia: Role of Tumor Necrosis Factor-{alpha}, NAD(P)H Oxidase, and Inducible Nitric Oxide Synthase Arterioscler. Thromb. Vasc. Biol., March 1, 2003; 23(3): 418 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. H. Bishopric and K. A. Webster Preventing Apoptosis With Thioredoxin: ASK Me How Circ. Res., June 28, 2002; 90(12): 1237 - 1239. [Full Text] [PDF]
|
|
|
|
|
|
T. Cyrus, L. X. Tang, J. Rokach, G. A. FitzGerald, and D. Pratico Lipid Peroxidation and Platelet Activation in Murine Atherosclerosis Circulation, October 16, 2001; 104(16): 1940 - 1945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Schecter, A. B. Berman, L. Yi, A. Mosoian, C. M. McManus, J. W. Berman, M. E. Klotman, and M. B. Taubman HIV envelope gp120 activates human arterial smooth muscle cells PNAS, August 10, 2001; (2001) 181328798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. J. Thannickal and B. L. Fanburg Reactive oxygen species in cell signaling Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1005 - L1028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Griendling, D. Sorescu, B. Lassegue, and M. Ushio-Fukai Modulation of Protein Kinase Activity and Gene Expression by Reactive Oxygen Species and Their Role in Vascular Physiology and Pathophysiology Arterioscler. Thromb. Vasc. Biol., October 1, 2000; 20(10): 2175 - 2183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Griendling, D. Sorescu, and M. Ushio-Fukai NAD(P)H Oxidase : Role in Cardiovascular Biology and Disease Circ. Res., March 17, 2000; 86(5): 494 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Rogers, J. M. Monnier, and H. S. Nick Tumor Necrosis Factor-alpha Selectively Induces MnSOD Expression via Mitochondria-to-Nucleus Signaling, whereas Interleukin-1beta Utilizes an Alternative Pathway J. Biol. Chem., June 1, 2001; 276(23): 20419 - 20427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Reddy, S. Hama, V. Grijalva, K. Hassan, R. Mottahedeh, G. Hough, D. J. Wadleigh, M. Navab, and A. M. Fogelman Mitogen-activated Protein Kinase Phosphatase 1 Activity Is Necessary for Oxidized Phospholipids to Induce Monocyte Chemotactic Activity in Human Aortic Endothelial Cells J. Biol. Chem., May 11, 2001; 276(20): 17030 - 17035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Schecter, A. B. Berman, L. Yi, A. Mosoian, C. M. McManus, J. W. Berman, M. E. Klotman, and M. B. Taubman HIV envelope gp120 activates human arterial smooth muscle cells PNAS, August 28, 2001; 98(18): 10142 - 10147. [Abstract] [Full Text] [PDF]
|
|