© 2000 American Heart Association, Inc.
Thrombosis |
Platelet-Leukocyte Cross Talk in Whole Blood
From the Department of Medicine (N.L., H.H., M.L., E.W.-J., P.H.), Division of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden, and the Division of Chemical Pathology (A.H.G.), University of Leicester, Glenfield Hospital, Leicester, UK.
Correspondence to Paul Hjemdahl, MD, PhD, Professor, Department of Medicine, Division of Clinical Pharmacology, Karolinska Hospital, SE-171 76 Stockholm, Sweden. E-mail Paul.Hjemdahl{at}ks.se
Abstract—Thrombosis and inflammation involve complex platelet-leukocyte interaction, the details of which are not fully elucidated. Therefore, we investigated cross talk between platelets and leukocytes in whole blood, under the following physiological conditions: at 37°C, with normal calcium concentrations, and with shear force. Platelet P-selectin and leukocyte CD11b expression were used to monitor platelet and leukocyte activation, respectively, and platelet-leukocyte aggregation (PLA) was analyzed. The leukocyte-specific agonist N-formyl-methionyl-leucyl-phenylalanine (10-6 mol/L) increased P-selectin–positive platelets from 2.5±0.1% to 5.1±0.6% (P<0.05). The increase was inhibited by either the platelet-activating factor (PAF) antagonist SR27417, the superoxide anion scavenger superoxide dismutase, the 5-lipoxygenase inhibitor Zileuton, or the 5-lipoxygenase–activating protein inhibitor MK-886, suggesting the involvement of PAF, superoxide anion, and 5-lipoxygenase products in leukocyte-induced platelet activation. The platelet-specific agonist collagen (1 µg/mL) increased leukocyte CD11b expression from 2.94±0.52 to 3.81±0.58 (P<0.05); this was not inhibited by the thromboxane A2 receptor antagonist ICI 192.605 or the PAF antagonist SR27417. Platelet P-selectin expression induced by N-formyl-methionyl-leucyl-phenylalanine and leukocyte CD11b expression induced by collagen could be suppressed by glycoprotein IIb/IIIa blockade or P-selectin blockade. This study documents platelet-leukocyte cross talk under conditions that mimic a physiological state and suggests that this involves multiple mediators and mechanisms. Furthermore, new evidence of integrin and selectin involvement in intracellular and intercellular signaling during platelet-leukocyte cross talk is provided.
Key Words: platelets • leukocytes • platelet-leukocyte aggregates • platelet-leukocyte cross talk • whole blood
This article has been cited by other articles:
 |
|
 |
|
  E. Pluskota, N. M. Woody, D. Szpak, C. M. Ballantyne, D. A. Soloviev, D. I. Simon, and E. F. Plow Expression, activation, and function of integrin {alpha}M{beta}2 (Mac-1) on neutrophil-derived microparticles Blood, September 15, 2008; 112(6): 2327 - 2335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. F. Bradfield, C. Scheiermann, S. Nourshargh, C. Ody, F. W. Luscinskas, G. E. Rainger, G. B. Nash, M. Miljkovic-Licina, M. Aurrand-Lions, and B. A. Imhof JAM-C regulates unidirectional monocyte transendothelial migration in inflammation Blood, October 1, 2007; 110(7): 2545 - 2555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Wang, H.-Y. Lin, M.-L. Cheng, and M.-K. Wong Chronic intermittent hypoxia modulates eosinophil- and neutrophil-platelet aggregation and inflammatory cytokine secretion caused by strenuous exercise in men J Appl Physiol, July 1, 2007; 103(1): 305 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schafer, U. Flierl, A. Kobsar, M. Eigenthaler, G. Ertl, and J. Bauersachs Soluble Guanylyl Cyclase Activation With HMR1766 Attenuates Platelet Activation in Diabetic Rats Arterioscler. Thromb. Vasc. Biol., December 1, 2006; 26(12): 2813 - 2818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Lu, Y. Chen, YinHuang, W. Li, and M. Jiang Venous Hypertension Induces Increased Platelet Reactivity and Accumulation in Patients with Chronic Venous Insufficiency Angiology, May 1, 2006; 57(3): 321 - 329. [Abstract] [PDF]
|
|
|
|
 |
|
 G. Hu, M. R. Salem, and G. J. Crystal Isoflurane Prevents Platelets from Enhancing Neutrophil-Induced Coronary Endothelial Dysfunction Anesth. Analg., November 1, 2005; 101(5): 1261 - 1268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D J H McCabe, P Harrison, I J Mackie, P S Sidhu, G Purdy, A S Lawrie, H Watt, S J Machin, and M M Brown Increased platelet count and leucocyte-platelet complex formation in acute symptomatic compared with asymptomatic severe carotid stenosis J. Neurol. Neurosurg. Psychiatry, September 1, 2005; 76(9): 1249 - 1254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mori, J. W. Salter, T. Vowinkel, C. F. Krieglstein, K. Y. Stokes, and D. N. Granger Molecular determinants of the prothrombogenic phenotype assumed by inflamed colonic venules Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G920 - G926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nemmar, B. Nemery, P. H. M. Hoet, N. Van Rooijen, and M. F. Hoylaerts Silica Particles Enhance Peripheral Thrombosis: Key Role of Lung Macrophage-Neutrophil Cross-Talk Am. J. Respir. Crit. Care Med., April 15, 2005; 171(8): 872 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Ritter, K. M. Stempel, B. M. Coull, and P. F. McDonagh Leukocyte-Platelet Aggregates in Rat Peripheral Blood After Ischemic Stroke and Reperfusion Biol Res Nurs, April 1, 2005; 6(4): 281 - 288. [Abstract] [PDF]
|
|
|
|
|
|
G.-S. Huang, C.-Y. Li, P.-C. Hsu, C.-S. Tsai, T.-C. Lin, and C.-S. Wong Sevoflurane Anesthesia Attenuates Adenosine Diphosphate-Induced P-Selectin Expression and Platelet-Leukocyte Conjugate Formation Anesth. Analg., October 1, 2004; 99(4): 1121 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schafer, N. J. Alp, S. Cai, C. A. Lygate, S. Neubauer, M. Eigenthaler, J. Bauersachs, and K. M. Channon Reduced Vascular NO Bioavailability in Diabetes Increases Platelet Activation In Vivo Arterioscler. Thromb. Vasc. Biol., September 1, 2004; 24(9): 1720 - 1726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Cooper, J. Russell, K. D. Chitman, M. C. Williams, R. E. Wolf, and D. N. Granger Leukocyte dependence of platelet adhesion in postcapillary venules Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1895 - H1900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schafer, F. Wiesmann, S. Neubauer, M. Eigenthaler, J. Bauersachs, and K. M. Channon Rapid Regulation of Platelet Activation In Vivo by Nitric Oxide Circulation, April 20, 2004; 109(15): 1819 - 1822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Koster, S. Huebler, F. Merkle, T. Hentschel, M. Grundel, T. Krabatsch, L. Tambeur, M. Praus, H. Habazettl, W. M. Kuebler, et al. Heparin-Level-Based Anticoagulation Management During Cardiopulmonary Bypass: A Pilot Investigation on the Effects of a Half-Dose Aprotinin Protocol on Postoperative Blood Loss and Hemostatic Activation and Inflammatory Response Anesth. Analg., February 1, 2004; 98(2): 285 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dalby, G. Montalescot, C. B. d. Sollier, E. Vicaut, T. Soulat, J.-P. Collet, R. Choussat, V. Gallois, G. Drobinski, L. Drouet, et al. Eptifibatide provides additional platelet inhibition in Non-ST-Elevation myocardial infarction patients already treated with aspirin and clopidogrel: Results of the platelet activity extinction in Non-Q-Wave myocardial infarction with aspirin, clopidogrel, and eptifibatide (PEACE) study J. Am. Coll. Cardiol., January 21, 2004; 43(2): 162 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cooper, K. D. Chitman, M. C. Williams, and D. N. Granger Time-dependent platelet-vessel wall interactions induced by intestinal ischemia-reperfusion Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G1027 - G1033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Quinn, T. V. Byzova, J. Qin, E. J. Topol, and E. F. Plow Integrin {alpha}IIb{beta}3 and Its Antagonism Arterioscler. Thromb. Vasc. Biol., June 1, 2003; 23(6): 945 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jayachandran, W. G. Owen, and V. M. Miller Effects of ovariectomy on aggregation, secretion, and metalloproteinases in porcine platelets Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1679 - H1685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Quinn, E. F. Plow, and E. J. Topol Platelet Glycoprotein IIb/IIIa Inhibitors: Recognition of a Two-Edged Sword? Circulation, July 16, 2002; 106(3): 379 - 385. [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. C. Ribeiro and I. M. B. Francischetti Platelet-activating-factor-hydrolyzing phospholipase C in the salivary glands and saliva of the mosquito Culex quinquefasciatus J. Exp. Biol., March 13, 2002; 204(22): 3887 - 3894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wallaschofski, M. Donne, M. Eigenthaler, B. Hentschel, R. Faber, H. Stepan, M. Koksch, and T. Lohmann PRL as a Novel Potent Cofactor for Platelet Aggregation J. Clin. Endocrinol. Metab., December 1, 2001; 86(12): 5912 - 5919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Vermylen, M. Hoylaerts, J. Arnout, D. P. Chew, D. L. Bhatt, E. J. Topol, and S. Sapp Increased Mortality With Long-Term Platelet Glycoprotein IIb/IIIa Antagonists: An Explanation? Response Circulation, November 13, 2001; 104 (20): e109 - e109. [Full Text] [PDF]
|
|