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(Arteriosclerosis, Thrombosis, and Vascular Biology. 1999;19:1708-1718.)
© 1999 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Coexistence of Oxidized Lipids and -Tocopherol in All Lipoprotein Density Fractions Isolated From Advanced Human Atherosclerotic Plaques

Xianwa Niu; Vivienne Zammit; Joanne M. Upston; Roger T. Dean; Roland Stocker

From the Biochemistry (X.N., V.Z., J.M.U., R.S.) and Cell Biology (R.T.D.) Groups, the Heart Research Institute, Camperdown, Australia.

Correspondence to Dr Roland Stocker, the Heart Research Institute, 145 Missenden Road, Camperdown, NSW 2050, Australia. E-mail r.stocker{at}hri.org.au

Abstract—After investigation of the contents and redox status of antioxidants and lipids in homogenates of both normal artery and atherosclerotic plaque, we now investigated them in the density fractions (very low, low, high, and protein fractions) of atherosclerotic plaque freshly obtained from carotid endarterectomy. By using the optimum extraction method (homogenization in carbonate buffer) and after density gradient ultracentrifugation, we isolated and characterized density fractions of plaque for apolipoproteins, size and contents of -tocopherol (-TOH), unesterified cholesterol, cholesteryl linoleate (Ch18:2), and hydroxides and hydroperoxides of Ch18:2, ie, Ch18:2-O(O)H. The distribution of apolipoproteins was more heterogeneous than that in the corresponding lipoproteins isolated from blood, and the majority of material in all plaque density fractions was present in large particles eluting in the void volume of gel-filtration columns. The content of unesterified cholesterol per unit of protein in low- and high-density fractions was 10-fold that in corresponding plasma lipoproteins. Low- and very-low-density fractions contained most of the lesion lipids and -TOH. Two to five percent of lesion Ch18:2 was present as Ch18:2-O(O)H and distributed more or less equally among all density fractions, yet the content of -TOH per unit of Ch18:2 was higher than that in corresponding plasma lipoproteins. These results demonstrate that -TOH and oxidized lipids coexist in all lesion density fractions, further supporting the notion that large proportions of lipids in lipoproteins of advanced stages of atherosclerosis are oxidized. However, although not ruling it out, our results do not support the suggestion that advanced stages of atherosclerosis are associated with gross deficiencies in the lipoproteins' vitamin E content.

Key Words: antioxidants • atherosclerosis • oxidative stress • vitamin E • lipid peroxidation

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