This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zibara, K. Articles by McGregor, J. L. Search for Related Content PubMed PubMed Citation Articles by Zibara, K. Articles by McGregor, J. L. Related Collections Animal models of human disease Physiological and pathological control of gene expression Smooth muscle proliferation and differentiation Mechanism of atherosclerosis/growth factors

(Arteriosclerosis, Thrombosis, and Vascular Biology. 1999;19:1650-1657.)
© 1999 American Heart Association, Inc.

Vascular Biology

Identification and Cloning of a New Gene (2A3-2), Homologous to Human Translational Elongation Factor, Upregulated in a Proliferating Rat Smooth Muscle Cell Line and in Carotid Hyperplasia

Kazem Zibara; Marie-Claude Bourdillon; Elza Chignier; Chantal Covacho; John L. McGregor

From INSERM Unit 331, Faculty of Medicine Laënnec, Lyon, France.

Correspondence to Dr K. Zibara, INSERM U331, Laënnec Medical School, 8 rue G. Paradin, 69372 Lyon cedex 08, France. E-mail zibara{at}laennec.univ-lyon1.fr

Abstract—Smooth muscle cells (SMCs), before migration and proliferation in the intima of the vessel wall, change from a normal contractile to a pathological proliferating phenotype. The molecular regulatory mechanisms implicated in such phenotypic changes remain poorly understood. In this study, using differential display, we have isolated for the first time a new gene (2A3-2) that is overexpressed in a rapidly proliferating, but not synthetic, rat SMC line. This was further confirmed by northern blot performed on the 2 cell types. Moreover, balloon catheter injury of rat carotids showed, by a virtual northern technique, an upregulation of this new gene in hyperplasia vessels. This new gene (2A3-2, 1.2 kb) was present in skeletal muscle, heart, aorta, lung, liver, kidney, and spleen. In addition, 5' rapid amplification of cDNA ends (5' RACE) allowed the cloning and sequencing of this 1.2-kb gene. Comparison of this newly identified gene sequence with data banks showed a strong homology to human and bovine mitochondrial translational elongation factor. The 2A3-2 gene, identified in this study, may play a vital role in the cascade of events implicated in switching SMC phenotype from a quiescent to a proliferate one.

Key Words: smooth muscle cell • differential display • virtual northern • rat carotid hyperplasia • cell culture • translational elongation factor

This article has been cited by other articles:

				P.A Henriksen and Y Kotelevtsev Application of gene expression profiling to cardiovascular disease Cardiovasc Res, April 1, 2002; 54(1): 16 - 24. [Abstract] [Full Text] [PDF]

				M.-C. Bourdillon, R. N. Poston, C. Covacho, E. Chignier, G. Bricca, and J. L. McGregor ICAM-1 Deficiency Reduces Atherosclerotic Lesions in Double-Knockout Mice (ApoE-/-/ICAM-1-/-) Fed a Fat or a Chow Diet Arterioscler Thromb Vasc Biol, December 1, 2000; 20(12): 2630 - 2635. [Abstract] [Full Text] [PDF]

				F. Oduol, J. Xu, O. Niaré, R. Natarajan, and K. D. Vernick Genes identified by an expression screen of the vector mosquito Anopheles gambiae display differential molecular immune response to malaria parasites and bacteria PNAS, September 22, 2000; (2000) 180060997. [Abstract] [Full Text]

				F. Oduol, J. Xu, O. Niare, R. Natarajan, and K. D. Vernick Genes identified by an expression screen of the vector mosquito Anopheles gambiae display differential molecular immune response to malaria parasites and bacteria PNAS, October 10, 2000; 97(21): 11397 - 11402. [Abstract] [Full Text] [PDF]