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(Arteriosclerosis, Thrombosis, and Vascular Biology. 1999;19:925-931.)
© 1999 American Heart Association, Inc.

Original Contributions

Uptake of 13-Hydroperoxylinoleic Acid by Cultured Cells

Nathalie Augé; Nalini Santanam; Natsuko Mori; Channa Keshava; Sampath Parthasarathy

From the Department of Gynecology and Obstetrics, Emory University, Atlanta, Ga. Current addresses for Nathalie Augé, PhD, Unité INSERM 466, Rangueil, Université de Médecine, 31403 Toulouse Cedex 4, France; and Channa Keshava, PhD, Toxicology and Molecular Biology Branch, HELD, CDC, NIOSH, M/S 3014, 1095 Willowdale Road, Morgantown, WV 26505.

Correspondence to Sampath Parthasarathy, PhD, Department of Gynecology and Obstetrics, Emory University, Atlanta, GA 30322. E-mail spartha{at}emory.edu

Abstract—Oxidized free fatty acids have profound effects on cultured cells. However, little is known about whether these effects depend on their uptake and metabolism by cells or primarily involve their interaction with cell-surface components. We determined the uptake and metabolism of unoxidized (linoleic or oleic acid) and oxidized linoleic acid (13-hydroperoxyoctadecadienoic acid, 13-HPODE) by endothelial cells, smooth muscle cells, and macrophages. We show that 13-HPODE is poorly taken up by cells. The levels of uptake were dependent on the cell type but were independent of the expression of CD36. 13-HPODE was also poorly used by microsomal lysophosphatidylcholine acyltransferase that is involved in the formation of phosphatidylcholine. Based on these results, we suggest that most of the biological effects of 13-HPODE and other oxidized free fatty acids on cells might involve a direct interaction with cell-surface components. Alternatively, very small amounts of oxidized free fatty acids that enter the cell may have effects, analogous to those of hormones or prostanoids.

Key Words: atherosclerosis • linoleic acid • 13-hydroperoxyoctadecadienoic acid • endothelial cells • macrophages

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