© 1999 American Heart Association, Inc.
Vascular Biology |
Lipoxygenase Products Increase Monocyte Adhesion to Human Aortic Endothelial Cells
From the Division of Cardiology (M.K.P., J.A.K., C.M.H., P.T.S., J.A.B., C.C.H.), University of California at Los Angeles, and the Department of Diabetes, Endocrinology, and Metabolism (R.N., J.L.N.), City of Hope National Medical Center, Duarte, Calif. J.A.K. is presently at the Department of Neurology, University of California Irvine and J.L.M. is presently at the Department of Endocrinology and Metabolism, University of Virginia at Charlottesville.
Correspondence to Catherine C. Hedrick, PhD, University of California Los Angeles, Division of Cardiology Room 47–123 CHS, 10833 Le Conte Ave, Los Angeles, CA 90095-1679. E-mail lhedrick{at}mednet.ucla.edu
Abstract—The development of atherosclerosis is accelerated in individuals with type 2 diabetes. Adhesion of monocytes to the vascular endothelium is a key initial step in atherogenesis. We have previously shown that monocyte adhesion to human aortic endothelial cells (HAECs) cultured long-term in high-glucose medium (25 mmol/L, 2 passages) is increased compared with cells grown in normal glucose (5 mmol/L). One potential mechanism for increased monocyte adhesion to HAECs under hyperglycemic conditions is via the 12-lipoxygenase (12-LO) pathway. In this study, we demonstrated in HAECs that the major LO metabolite of arachidonic acid was the 12-LO product, 12(S)-hydroxyeicosatetraenoic acid [12(S)-HETE], which was increased severalfold in HAECs cultured under high-glucose conditions. Furthermore, treatment of HAECs with 12(S)-HETE induced monocyte, but not neutrophil, adhesion an average of 3-fold (range of 1.5- to 5-fold) compared with untreated cells (75±5 versus 26±1 monocytes per field, respectively, P<0.001). Expression of the adhesion molecules vascular cell adhesion molecule-1, E-selectin, and intercellular adhesion molecule-1 was not significantly increased. However, both glucose and 12(S)-HETE induced a 60% increase in HAEC surface expression of connecting segment-1 (ie, CS-1) fibronectin, a ligand for very late–acting antigen-4 (VLA-4). The antibodies used to block monocyte integrin VLA-4 and leukocyte function–related antigen-1, a monocytic counterreceptor for intercellular adhesion molecule-1, inhibited the ability of both 12-LO products and high glucose to induce monocyte adhesion. These results definitively demonstrate for the first time in HAECs that the 12-LO pathway can induce monocyte–endothelial cell interaction and that the effects of glucose may be mediated, at least in part, through this pathway. Thus, these results suggest that the 12-LO pathway may play a role in the increased susceptibility of diabetics to atherosclerosis.
Key Words: 12(S)-hydroxyeicosatetraenoic acid • endothelium • hyperglycemia • lipoxygenase • monocytes
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