This Article Full Text Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Serdyuk, A. P. Articles by Morton, R. E. Search for Related Content PubMed PubMed Citation Articles by Serdyuk, A. P. Articles by Morton, R. E.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 1997;17:1716-1724.)
© 1997 American Heart Association, Inc.

Articles

Lipid Transfer Inhibitor Protein Activity Deficiency in Normolipidemic Uremic Patients on Continuous Ambulatory Peritoneal Dialysis

Anatole P. Serdyuk; ; Richard E. Morton

From the Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio.

Correspondence to Dr Richard E. Morton, Department of Cell Biology, Lerner Research Institute, NC 10, Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195. E-mail mortonr{at}cesmtp.ccf.org

Abstract We previously demonstrated that lipid transfer inhibitor protein (LTIP) is a potent modifier of lipid transfer protein (LTP) function in vitro. Based on these studies, we proposed that LTIP activity is an important determinant of lipoprotein size and composition, which leads to a stimulation of reverse cholesterol transport. To further evaluate this hypothesis, we have studied a normolipidemic, uremic patient population undergoing continuous ambulatory peritoneal dialysis (CAPD) that is deficient in LTIP activity (<18% of control). LDL from CAPD plasma was triglyceride enriched; the diameters of both CAPD LDL and HDL were increased and CAPD HDL was dominated by the largest subfraction, HDL_2b. In CAPD patients, the plasma cholesterol esterification rate was only 61% of control; this decrease was due mainly to the poor reactivity of CAPD lipoproteins. CAPD lipoprotein-deficient plasma promoted twofold greater transfer of radiolabeled cholesteryl ester (CE) between standard lipoproteins than control, although LTP itself was increased only 39%. This twofold increase was not equally expressed among individual lipoprotein classes; CE transfers involving LDL were increased 2.4-fold, whereas those not involving LDL were increased only 50%. In whole plasma, CE net mass transfer to VLDL was slightly increased in CAPD plasma; relative to their CE content, control HDL contributed twofold more CE mass to VLDL than control LDL, but in CAPD plasma this preferential transfer of CE from HDL was absent. Collectively, the aberrations in CAPD lipoprotein composition and metabolism are consistent with the hypothesized role of LTIP. The data further support the role of LTIP in modulating the participation of HDL in CE mass transfers to VLDL. This is the first report of LTIP activity deficiency in humans.

Key Words: lipid transfer inhibitor protein • lipid transfer protein • continuous ambulatory peritoneal dialysis • lecithin:cholesterol acyltransferase

This article has been cited by other articles:

				W. R. Lagor, R. J. Brown, S.-A. Toh, J. S. Millar, I. V. Fuki, M. de la Llera-Moya, T. Yuen, G. Rothblat, J. T. Billheimer, and D. J. Rader Overexpression of Apolipoprotein F Reduces HDL Cholesterol Levels In Vivo Arterioscler. Thromb. Vasc. Biol., January 1, 2009; 29(1): 40 - 46. [Abstract] [Full Text] [PDF]

				R. E. Morton, H. M. Gnizak, D. J. Greene, K.-H. Cho, and V. M. Paromov Lipid transfer inhibitor protein (apolipoprotein F) concentration in normolipidemic and hyperlipidemic subjects J. Lipid Res., January 1, 2008; 49(1): 127 - 135. [Abstract] [Full Text] [PDF]

				R. E. Morton and D. J. Greene CETP and lipid transfer inhibitor protein are uniquely affected by the negative charge density of the lipid and protein domains of LDL J. Lipid Res., December 1, 2003; 44(12): 2287 - 2296. [Abstract] [Full Text] [PDF]

				R. E. Morton and D. J. Greene The surface cholesteryl ester content of donor and acceptor particles regulates CETP: a liposome-based approach to assess the substrate properties of lipoproteins J. Lipid Res., July 1, 2003; 44(7): 1364 - 1372. [Abstract] [Full Text] [PDF]

				J. W. Skeggs and R. E. Morton LDL and HDL enriched in triglyceride promote abnormal cholesterol transport J. Lipid Res., August 1, 2002; 43(8): 1264 - 1274. [Abstract] [Full Text] [PDF]

				R. E. Morton, V. Nunes, L. Izem, and E. Quintao Markedly Elevated Lipid Transfer Inhibitor Protein in Hypercholesterolemic Subjects Is Mitigated by Plasma Triglyceride Levels Arterioscler. Thromb. Vasc. Biol., October 1, 2001; 21(10): 1642 - 1649. [Abstract] [Full Text] [PDF]

				A. P. Serdyuk and R. E. Morton Lipid Transfer Inhibitor Protein Defines the Participation of Lipoproteins in Lipid Transfer Reactions : CETP Has No Preference for Cholesteryl Esters in HDL Versus LDL Arterioscler. Thromb. Vasc. Biol., March 1, 1999; 19(3): 718 - 726. [Abstract] [Full Text] [PDF]

				X. Wang, D. M. Driscoll, and R. E. Morton Molecular Cloning and Expression of Lipid Transfer Inhibitor Protein Reveals Its Identity with Apolipoprotein F J. Biol. Chem., January 15, 1999; 274(3): 1814 - 1820. [Abstract] [Full Text] [PDF]