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(Arteriosclerosis, Thrombosis, and Vascular Biology. 1997;17:1258-1266.)
© 1997 American Heart Association, Inc.

Articles

Lysophosphatidylcholine Promotes Cholesterol Efflux From Mouse Macrophage Foam Cells

Seijiro Hara; Tsutomu Shike; Nobuo Takasu; ; Takuji Mizui

From Discovery Research Laboratories II, Shionogi & Co, Ltd, Osaka, Japan.

Correspondence to Seijiro Hara, PhD, Developmental Research Laboratories, Shionogi & Co, Ltd, 3-1-1, Futaba-Cho, Toyonaka, Osaka 561, Japan. E-mail seijiro.hara{at}shionogi.co.jp

Abstract We examined the effects of lysophosphatidylcholine (lyso-PC) on promoting cholesterol efflux from macrophage foam cells. Mouse peritoneal macrophages were converted to foam cells by incubation with [³H]cholesteryl linoleate–labeled or unlabeled acetyl-LDL. When these cells were incubated with lyso-PC, [³H]cholesterol release was promoted in relation to both dose and time, and cellular cholesterol mass was decreased, while medium cholesterol mass was increased. These cholesterol efflux–promotive effects of lyso-PC were confirmed by the fact that the lyso-PC–treated cells showed less oil red O staining than the control cells. ApoE secretion, estimated by Western blotting of the medium, was also augmented by lyso-PC. Both the cholesterol and apoE released by lyso-PC treatment were floated by ultracentrifugation of the medium after its density had been adjusted to 1.210 g/mL. By electron microscopic analysis, vesicular lipoproteins were observed in ultracentrifugally concentrated conditioned medium of lyso-PC. Monensin, a protein secretion inhibitor, effectively inhibited [³H]cholesterol release induced by lyso-PC but not by apoA-I. These results suggest that lyso-PC may inhibit the development of atherosclerosis or enhance its regression by stimulating cholesterol efflux from macrophage foam cells.

Key Words: lysophosphatidylcholine • cholesterol efflux • apoE

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