© 1997 American Heart Association, Inc.
Articles |
Association of Apolipoprotein Genetic Polymorphisms With Plasma Cholesterol in a Japanese Rural Population
The Shibata Study
From the Department of Epidemiology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan (M.M.Z., T.Y., H.T.); the Division of Clinical Nutrition, National Institute of Health and Nutrition, Tokyo, Japan (S.I.); the Division of Adult Health Science, National Institute of Health and Nutrition, Tokyo, Japan (N.Y.); and the Department of Public Health, Osaka City University School of Medicine, Osaka, Japan (C.D.).
Correspondence to Dr Mohammad Mostafa Zaman, Department of Epidemiology, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kandasurugadai, Chiyoda-ku, Tokyo, 101 Japan. E-mail zaman.epi{at}mri.tmd.ac.jp
Abstract The association between apolipoprotein (apo) genetic
polymorphisms and lipid phenotypes remains to be determined
because such studies have reported contradictory results. We have
measured plasma total cholesterol (TC) and HDL
cholesterol (HDL-C) in a cross-sectional sample of 1328
(462 men and 866 women) Japanese (aged 40 to 80 years) and
analyzed their DNA for the following genotypes:
apoA1-C3 Msp I and Sst I sites; apoB
signal peptide insertion/deletion, Xba I site and 3'
variable number of tandem repeats (VNTR); and apoE. Using
multivariate analyses (ANOVA) we found that (1)
the polymorphisms of apoA1-C3 and apoB genes are not significantly
associated with TC or HDL-C and (2) the polymorphism of the apoE
gene is significantly related with TC and the TC:HDL-C ratio. The 
2
carriers have lower levels of TC and a lower TC:HDL-C ratio, 3
carriers have intermediate levels, and 4 carriers have higher
levels. These findings held whether sexes were analyzed
separately or together. Although an opposite trend in HDL-C levels was
observed, it did not reach the level of statistical significance.
Multiple regression analyses have shown that apoE
polymorphism accounts for about 2.3% of the variation in TC and
TC:HDL-C ratio irrespective of sex. When the subjects are divided into
two groups according to their age (
59 and 
60 years old), the effect
of apoE alleles on serum cholesterols appears to be
diluted in the younger age group whereas it appears to be accentuated
in the older group for both sexes. Our findings suggest that among the
apo genetic polymorphisms examined, the apoE gene may be considered
as a possible candidate for the "high-risk" strategy of
atherosclerosis prevention in the Japanese
population.
Key Words: apolipoprotein genes • plasma cholesterol • Japanese population
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