This Article Full Text Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yasunari, K. Articles by Yoshikawa, J. Search for Related Content PubMed PubMed Citation Articles by Yasunari, K. Articles by Yoshikawa, J. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB DOPAMINE

(Arteriosclerosis, Thrombosis, and Vascular Biology. 1997;17:3164-3173.)
© 1997 American Heart Association, Inc.

Articles

Dopamine as a Novel Antimigration and Antiproliferative Factor of Vascular Smooth Muscle Cells Through Dopamine D1-Like Receptors

Kenichi Yasunari; Masakazu Kohno; Tadayoshi Hasuma; Takeshi Horio; Hiroaki Kano; Koji Yokokawa; Mieko Minami; ; Junichi Yoshikawa

From the The First Department of Internal Medicine (K.Y., M.K., T.Ho., H.K., K.Y., M.M., J.Y.) and The Second Department of Biochemistry (T.Ha.), Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka, Japan.

Correspondence to Kenichi Yasunari, MD, The First Department of Internal Medicine, Osaka City University Medical School, 1-5-7 Asahi-machi, Abeno-ku, Osaka 545, Japan.

Abstract Vascular smooth muscle cell (VSMC) migration and proliferation are believed to play key roles in atherosclerosis. To elucidate the role of vascular dopamine D1-like receptors in atherosclerosis, the effects of dopamine and specific D1-like agonists SKF 38393 and YM 435 on platelet-derived growth factor (PDGF) BB-mediated VSMC migration and proliferation were studied. We observed that cells stimulated by PDGF-BB (5 ng/mL), showed increased migration and proliferation. These effects were prevented by coincubation with dopamine, SKF 38393, or YM 435 (1 to 10 µmol/L), and this prevention was reversed by Sch 23390 (1 to 10 µmol/l), a specific D1-like antagonist. These actions are mimicked by forskolin (1 to 10 µmol/L), a direct activator of adenylate cyclase and 8-bromo-cAMP at 0.1 to 1 mmol/L and are blocked by a specific protein kinase A inhibitor, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline-sulfonamide (H 89), but not blocked by its negative control, N-[2-(N-formyl)-p-chlorociannamylamino)ethyl]-5-isoquinoline sulfonamide (H 85). PDGF-BB (5 ng/mL)-mediated activation of phospholipase D, protein kinase C, and mitogen activated protein kinase activity were significantly suppressed by coincubation with dopamine. These results suggest that vascular D1-like receptor agonists inhibit migration and proliferation of VSMC, possibly through protein kinase A activation and suppression of activated phospholipase D, protein kinase C, and mitogen-activated protein kinase activity.

Key Words: dopamine • vascular smooth muscle • migration • proliferation

This article has been cited by other articles:

				Z. Li, C. Yu, Y. Han, H. Ren, W. Shi, C. Fu, D. He, L. Huang, C. Yang, X. Wang, et al. Inhibitory effect of D1-like and D3 dopamine receptors on norepinephrine-induced proliferation in vascular smooth muscle cells Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2761 - H2768. [Abstract] [Full Text] [PDF]

				C. Zeng, I. Armando, Y. Luo, G. M. Eisner, R. A. Felder, and P. A. Jose Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H551 - H569. [Abstract] [Full Text] [PDF]

				W. T. Gerthoffer Mechanisms of Vascular Smooth Muscle Cell Migration Circ. Res., March 16, 2007; 100(5): 607 - 621. [Abstract] [Full Text] [PDF]

				D. Chalothorn, H. Zhang, J. A. Clayton, S. A. Thomas, and J. E. Faber Catecholamines augment collateral vessel growth and angiogenesis in hindlimb ischemia Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H947 - H959. [Abstract] [Full Text] [PDF]

				N. Ohtani, T. Goto, C. Waeber, and P. G. Bhide Dopamine Modulates Cell Cycle in the Lateral Ganglionic Eminence J. Neurosci., April 1, 2003; 23(7): 2840 - 2850. [Abstract] [Full Text] [PDF]

				K. Yasunari, K. Maeda, M. Minami, and J. Yoshikawa HMG-CoA Reductase Inhibitors Prevent Migration of Human Coronary Smooth Muscle Cells Through Suppression of Increase in Oxidative Stress Arterioscler Thromb Vasc Biol, June 1, 2001; 21(6): 937 - 942. [Abstract] [Full Text] [PDF]

				K. Yasunari, M. Kohno, H. Kano, M. Minami, and J. Yoshikawa Dopamine as a Novel Antioxidative Agent for Rat Vascular Smooth Muscle Cells Through Dopamine D1-Like Receptors Circulation, May 16, 2000; 101(19): 2302 - 2308. [Abstract] [Full Text] [PDF]