© 1997 American Heart Association, Inc.
Articles |
Monounsaturated and Polyunsaturated n-6 Fatty Acid–Enriched Diets Modify LDL Oxidation and Decrease Human Coronary Smooth Muscle Cell DNA Synthesis
From the Lipid Research Unit, Department of Medicine, Fundación Jiménez Díaz, Universidad Autónoma Madrid (P.M., R.A., C.L., M.d.O.), and the Cardiovascular Research Center, Consejo Superior de Investigaciones Científicas–Hospital de la Sta. Cruz y San Pablo, Universidad Autónoma Barcelona (O.V., L.B.), Spain.
Correspondence to Prof Lina Badimon, Cardiovascular Unit, CSIC, Jordi Girona, 18-26 # Lab. 623, 08034 Barcelona, Spain. E-mail: lbmucv{at}cid.csic.es.
Abstract Proliferation of smooth muscle cells (SMCs) plays an important role in atherosclerotic lesion progression. The purpose of this investigation was to examine the effect of diets differing in fatty acid composition on human coronary SMC entry in the cell proliferation cycle. Twenty-four healthy men and women were placed on four consecutive diets lasting 5 weeks each: (1) saturated fatty acid (SFA)–rich diet with palm oil; (2) monounsaturated fatty acid (MUFA)–rich diet with olive oil; (3) polyunsaturated fatty acid (PUFA) n-6–rich diet with sunflower oil; and (4) PUFA n-3–rich diet (3.8 g/d). All diets supplied 35% of calories as fat. Compared with the SFA diet, all unsaturated diets reduced LDL cholesterol. Resistance of LDL to oxidative modification was significantly increased during the MUFA period (P<.05). Human coronary SMCs were cultured and induced by sera derived from the different groups. 3H-Thymidine incorporation into doubling DNA was significantly (P<.01) reduced during the MUFA and PUFA n-6 periods but not during the PUFA n-3 diet with respect to the SFA diet. This effect was more pronounced in women than in men. In conclusion, the MUFA-enriched diet reduced SMC DNA synthesis and LDL levels and protected LDL from oxidation. Therefore, these combined effects suggest that an oleic acid–rich Mediterranean diet could be better than PUFA (n-6)– or PUFA (n-3)–rich diets in the prevention of atherosclerosis.
Key Words: monounsaturated fatty acids • polyunsaturated fatty acids • lipoproteins • lipid oxidation • smooth muscle cells • atherosclerosis
This article has been cited by other articles:
 |
|
 |
|
  S. Rodenas, S. Rodriguez-Gil, M. C. Merinero, and F. J. Sanchez-Muniz Dietary Exchange of an Olive Oil and Sunflower Oil Blend for Extra Virgin Olive Oil Decreases the Estimate Cardiovascular Risk and LDL and Apolipoprotein AII Concentrations in Postmenopausal Women J. Am. Coll. Nutr., October 1, 2005; 24(5): 361 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Weinbrenner, M. Fito, R. d. l. Torre, G. T. Saez, P. Rijken, C. Tormos, S. Coolen, M. F. Albaladejo, S. Abanades, H. Schroder, et al. Olive Oils High in Phenolic Compounds Modulate Oxidative/Antioxidative Status in Men J. Nutr., September 1, 2004; 134(9): 2314 - 2321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Fernandez-Jarne, E Martinez-Losa, M Prado-Santamaria, C Brugarolas-Brufau, M Serrano-Martinez, and M. Martinez-Gonzalez Risk of first non-fatal myocardial infarction negatively associated with olive oil consumption: a case-control study in Spain Int. J. Epidemiol., April 1, 2002; 31(2): 474 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sugano, S. Sawada, K. Tsuchida, N. Makino, and M. Kamada Low density lipoproteins develop resistance to oxidative modification due to inhibition of cholesteryl ester transfer protein by a monoclonal antibody J. Lipid Res., January 1, 2000; 41(1): 126 - 133. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. A Papadakis, D. P Mikhailidis, and A. F Winder Lipids and stroke: neglect of a useful preventive measure? Cardiovasc Res, November 1, 1998; 40(2): 265 - 271. [Abstract] [Full Text] [PDF]
|
|