© 1996 American Heart Association, Inc.
Articles |
Interleukin-8 Production by Macrophages From Atheromatous Plaques
the Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, Victoria, Australia.
Correspondence to Dr J. Apostolopoulos, Department of Medicine, Level 5, E-Block, Monash Medical Centre, 246 Clayton Rd, Clayton, 3168, Victoria, Australia. E-mail Jim.Apostolopoulos@med.monash.edu.au.
Interleukin-8 (IL-8) is a chemotactic peptide produced by macrophages that may be involved in the recruitment of inflammatory cells into atherosclerotic plaques. In vitro, IL-8 production by macrophages isolated from carotid plaques (1240±510 pg·105 cells-1·24 h-1, mean±SEM, n=6) and noncarotid plaques (4312±1588 pg·105 cells-1·24 h-1, n=9) was significantly greater than IL-8 production by blood monocytes isolated from the same patients (526±278 pg·105 cells-1·24 h-1, n=6, P<.05 and 726±384 pg·105 cells-1·24 h-1, n=9, P<.01, respectively). IL-8 produced by atherosclerotic macrophages was demonstrated to be biologically active in a neutrophil chemotaxis assay. IL-8 mRNA was detectable in plaque macrophages and blood monocytes from these patients, but blood monocytes from normal donors did not exhibit detectable IL-8 mRNA. IL-8 mRNA was localized in macrophage-rich areas of atherosclerotic plaques by in situ hybridization. These studies demonstrate that macrophages from atherosclerotic plaques show an enhanced capacity to produce IL-8 compared with normal and patient blood monocytes and that macrophages are a major site of IL-8 mRNA production in atherosclerotic plaques. These results provide further evidence for a proinflammatory role for macrophages in atherosclerosis.
Key Words: interleukin-8 • atherosclerosis • macrophage • human
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