Arteriosclerosis, Thrombosis, and Vascular Biology. 1996;16:600-605
© 1996 American Heart Association, Inc.
Articles |
Lysophosphatidylcholine Potentiates the Mitogenic Activity of Modified LDL for Human Monocyte–Derived Macrophages
From the Departments of Biochemistry (A.M., H.H., S.H.) and Metabolic Medicine (M. Sakai, Y.S., T.M., S.K., M. Shichiri), Kumamoto University School of Medicine, Kumamoto, Japan.
Correspondence to Seikoh Horiuchi, MD, PhD, Department of Biochemistry, Kumamoto University School of Medicine, Honjo 2-2-1, Kumamoto 860, Japan.
Abstract The growth of murine peritoneal macrophages is induced by oxidized LDL (Ox-LDL), and lysophosphatidylcholine (lysoPC) plays an important role in its mitogenic activity. In the present study, Ox-LDL–induced macrophage growth was examined with human monocyte–derived macrophages. The cell growth of human macrophages was significantly induced by Ox-LDL but not by acetylated LDL (acetyl-LDL). The treatment of acetyl-LDL with phospholipase A2, however, led to a marked increase in its mitogenic activity, with a concomitant conversion of 75% of its phospholipids to lysoPC. The growth-stimulating activity became positive only when both acetyl-LDL and lysoPC were coincubated, although neither of them exhibited cell growth–promoting activity. These results suggest that Ox-LDL could stimulate the growth of human monocyte–derived macrophages, and lysoPC may play an essential role in the mitogenic activity of Ox-LDL.
Key Words: human monocyte–derived macrophage • foam cell • oxidized LDL • lysophosphatidylcholine • scavenger receptor
This article has been cited by other articles:
 |
|
 |
|
  B. W. Parks, G. P. Gambill, A. J. Lusis, and J. H. S. Kabarowski Loss of G2A promotes macrophage accumulation in atherosclerotic lesions of low density lipoprotein receptor-deficient mice J. Lipid Res., July 1, 2005; 46(7): 1405 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Fukuhara-Takaki, M. Sakai, Y.-i. Sakamoto, M. Takeya, and S. Horiuchi Expression of Class A Scavenger Receptor Is Enhanced by High Glucose in Vitro and under Diabetic Conditions in Vivo: ONE MECHANISM FOR AN INCREASED RATE OF ATHEROSCLEROSIS IN DIABETES J. Biol. Chem., February 4, 2005; 280(5): 3355 - 3364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Rebai, J. Le Petit-Thevenin, N. Bruneau, D. Lombardo, and A. Verine In Vitro Angiogenic Effects of Pancreatic Bile Salt-Dependent Lipase Arterioscler. Thromb. Vasc. Biol., February 1, 2005; 25(2): 359 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Rikitake, K.-i. Hirata, T. Yamashita, K. Iwai, S. Kobayashi, H. Itoh, M. Ozaki, J. Ejiri, M. Shiomi, N. Inoue, et al. Expression of G2A, a Receptor for Lysophosphatidylcholine, by Macrophages in Murine, Rabbit, and Human Atherosclerotic Plaques Arterioscler. Thromb. Vasc. Biol., December 1, 2002; 22(12): 2049 - 2053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Hundal, B. S. Salh, J. W. Schrader, A. Gomez-Munoz, V. Duronio, and U. P. Steinbrecher Oxidized low density lipoprotein inhibits macrophage apoptosis through activation of the PI 3-kinase/PKB pathway J. Lipid Res., September 1, 2001; 42(9): 1483 - 1491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Aikawa, E. Rabkin, S. Sugiyama, S. J. Voglic, Y. Fukumoto, Y. Furukawa, M. Shiomi, F. J. Schoen, and P. Libby An HMG-CoA Reductase Inhibitor, Cerivastatin, Suppresses Growth of Macrophages Expressing Matrix Metalloproteinases and Tissue Factor In Vivo and In Vitro Circulation, January 16, 2001; 103(2): 276 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Hamilton, G. Whitty, and W. Jessup Oxidized LDL Can Promote Human Monocyte Survival Arterioscler. Thromb. Vasc. Biol., October 1, 2000; 20 (10): 2329 - 2331. [Full Text] [PDF]
|
|
|
|
|
|
Y. Rikitake, S. Kawashima, T. Yamashita, T. Ueyama, S. Ishido, H. Hotta, K.-i. Hirata, and M. Yokoyama Lysophosphatidylcholine Inhibits Endothelial Cell Migration and Proliferation via Inhibition of the Extracellular Signal-Regulated Kinase Pathway Arterioscler. Thromb. Vasc. Biol., April 1, 2000; 20(4): 1006 - 1012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Biwa, M. Sakai, T. Matsumura, S. Kobori, K. Kaneko, A. Miyazaki, H. Hakamata, S. Horiuchi, and M. Shichiri Sites of Action of Protein Kinase C and Phosphatidylinositol 3-Kinase Are Distinct in Oxidized Low Density Lipoprotein-induced Macrophage Proliferation J. Biol. Chem., February 25, 2000; 275(8): 5810 - 5816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. J. de Winther, K. W. van Dijk, L. M. Havekes, and M. H. Hofker Macrophage Scavenger Receptor Class A : A Multifunctional Receptor in Atherosclerosis Arterioscler. Thromb. Vasc. Biol., February 1, 2000; 20(2): 290 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sakashita, A. Miyazaki, M. Takeya, S. Horiuchi, C. C. Y. Chang, T.-Y. Chang, and K. Takahashi Localization of Human Acyl-Coenzyme A:Cholesterol Acyltransferase-1 (ACAT-1) in Macrophages and in Various Tissues Am. J. Pathol., January 1, 2000; 156(1): 227 - 236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsumura, M. Sakai, K. Matsuda, N. Furukawa, K. Kaneko, and M. Shichiri Cis-acting DNA Elements of Mouse Granulocyte/Macrophage Colony-stimulating Factor Gene Responsive to Oxidized Low Density Lipoprotein J. Biol. Chem., December 31, 1999; 274(53): 37665 - 37672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sakai, T. Biwa, T. Matsumura, T. Takemura, H. Matsuda, Y. Anami, T. Sasahara, S. Kobori, and M. Shichiri Glucocorticoid Inhibits Oxidized LDL-Induced Macrophage Growth by Suppressing the Expression of Granulocyte/Macrophage Colony-Stimulating Factor Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1726 - 1733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. E. D. van den Eijnden, J. T. van Noort, L. Hollaar, A. van der Laarse, and R. M. Bertina Cholesterol or Triglyceride Loading of Human Monocyte-Derived Macrophages by Incubation With Modified Lipoproteins Does Not Induce Tissue Factor Expression Arterioscler. Thromb. Vasc. Biol., February 1, 1999; 19(2): 384 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sata and K. Walsh Endothelial Cell Apoptosis Induced by Oxidized LDL Is Associated with the Down-regulation of the Cellular Caspase Inhibitor FLIP J. Biol. Chem., December 11, 1998; 273(50): 33103 - 33106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Biwa, H. Hakamata, M. Sakai, A. Miyazaki, H. Suzuki, T. Kodama, M. Shichiri, and S. Horiuchi Induction of Murine Macrophage Growth by Oxidized Low Density Lipoprotein Is Mediated by Granulocyte Macrophage Colony-stimulating Factor J. Biol. Chem., October 23, 1998; 273(43): 28305 - 28313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hakamata, A. Miyazaki, M. Sakai, H. Matsuda, H. Suzuki, T. Kodama, and S. Horiuchi Isolation of macrophage-like cell mutants resistant to the cytotoxic effect of oxidized low density lipoprotein J. Lipid Res., March 1, 1998; 39(3): 482 - 494. [Abstract] [Full Text]
|
|
|
|
|
|
W. Li, X. M. Yuan, A. G. Olsson, and U. T. Brunk Uptake of Oxidized LDL by Macrophages Results in Partial Lysosomal Enzyme Inactivation and Relocation Arterioscler. Thromb. Vasc. Biol., February 1, 1998; 18(2): 177 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsumura, M. Sakai, S. Kobori, T. Biwa, T. Takemura, H. Matsuda, H. Hakamata, S. Horiuchi, and M. Shichiri Two Intracellular Signaling Pathways for Activation of Protein Kinase C Are Involved in Oxidized Low-Density Lipoprotein–Induced Macrophage Growth Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 3013 - 3020. [Abstract] [Full Text]
|
|
|
|
|
|
M. Sakai, A. Miyazaki, H. Hakamata, T. Kodama, H. Suzuki, S. Kobori, M. Shichiri, and S. Horiuchi The Scavenger Receptor Serves as a Route for Internalization of Lysophosphatidylcholine in Oxidized Low Density Lipoprotein-induced Macrophage Proliferation J. Biol. Chem., November 1, 1996; 271(44): 27346 - 27352. [Abstract] [Full Text] [PDF]
|
|