© 1995 American Heart Association, Inc.
Articles |
Presence and Formation of `Free Apolipoprotein A-I–Like' Particles in Human Plasma
From Louisiana State University Medical Center, Division of Lipoprotein Metabolism and Pathophysiology, Department of Physiology, New Orleans, La.
Correspondence to Paul S. Roheim, MD, Louisiana State University Medical Center, Department of Physiology, 1542 Tulane Ave, New Orleans, LA 70112-2822.
Abstract The influence of dilution on apolipoprotein (apo)
A-I–containing subpopulations was studied in human plasma. Agarose
electrophoresis and two-dimensional agarose nondenaturing gradient
polyacrylamide gel electrophoresis were used. Both in one- and
two-dimensional electrophoresis, an increase of charge was observed
that resulted in an increase of subpopulations with pre-
mobility.
Dilution of plasma also resulted in a decrease in the size of apo
A-I–containing pre-ß1 subpopulations. The existence of
smaller pre-ß1 particles was confirmed by subjecting
undiluted and 8x diluted plasma to 3% to 16% nondenaturing gradient
gel electrophoresis for 4 hours. In addition to the generally observed
pre-ß1 subpopulations, smaller particles similar in size
to the free apo A-I were detected even in the undiluted plasma. During
dilution, the proportion of larger pre-ß1 particles
decreased while the smaller ones increased, and in 8x diluted plasma,
almost all the pre-ß1 was present in smaller sizes.
Using 3% to 35% nondenaturing polyacrylamide gels run for 24
hours, no pre-ß1 particles could be detected in 8x
diluted plasma because the small pre-ß1 electrophoresed
out. These studies show that pre-ß1 particles can be
converted to smaller ones during dilution. It also was demonstrated
that "free apo A-I–like" pre-ß1 particles are
present in undiluted plasma. The presence of these particles may
have important physiological and
pathophysiological functions.
Key Words: apolipoproteins • plasma • electrophoresis
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