This Article Full Text Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, Y. Articles by Assmann, G. Search for Related Content PubMed PubMed Citation Articles by Huang, Y. Articles by Assmann, G.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 1995;15:1412-1418.)
© 1995 American Heart Association, Inc.

Articles

Cholesterol Efflux, Cholesterol Esterification, and Cholesteryl Ester Transfer by LpA-I and LpA-I/A-II in Native Plasma

Yadong Huang; Arnold von Eckardstein; Shili Wu; Gerd Assmann

From the Institut für Arterioskleroseforschung an der Universität Münster (Y.H., S.W., G.A.) and the Institut für Klinische Chemie und Laboratoriumsmedizin, Zentrallaboratorium, Westfälische Wilhelms-Universität Münster (A. von E., G.A.), Germany.

Correspondence to Yadong Huang, Institut für Arterioskleroseforschung an der Universität Münster, Domagkstr 3, D-48149 Münster, Federal Republic of Germany.

Abstract HDLs encompass structurally heterogeneous particles that fulfill specific functions in reverse cholesterol transport. Two-dimensional nondenaturing polyacrylamide gradient gel electrophoresis (2D-PAGGE) of normal plasma and subsequent immunoblotting with anti–apolipoprotein (apo) A-I antibodies differentiates an abundant particle with electrophoretic -mobility and less abundant particles with electrophoretic pre-ß-mobility (preß₁–LpA-I, preß₂–LpA-I, preß₃–LpA-I). Immunodetection with anti–apoA-II antibodies identifies a single particle with -mobility. To differentiate -migrating HDL without apo A-II (–LpA-I) from those with apoA-II (–LpA-I/A-II), we combined 2D-PAGGE with immunoadsorption of apoA-II. Incubation of plasma with [³H]cholesterol-labeled fibroblasts in combination with immunosubtracting 2D-PAGGE allowed us to analyze the role of –LpA-I and –LpA-I/A-II in the uptake and esterification of cell-derived cholesterol in native plasma. Depending on the duration of incubations with cells, -LpA-I took up two to four times more [³H]cholesterol than –LpA-I/A-II. Irrespective of the duration of incubation, two to three times more [³H]cholesteryl esters accumulated in –LpA-I than in –LpA-I/A-II. Subsequent incubations in the presence of an inhibitor of lecithin:cholesterol acyltransferase led to preferential accumulation of [³H]cholesteryl esters in –LpA-I/A-II. In conclusion, our data indicate that –LpA-I is more effective than –LpA-I/A-II in both uptake and esterification of cell-derived cholesterol. Moreover, –LpA-I/A-II appears to accumulate cholesteryl esters, at least partially, from –LpA-I.

Key Words: apoA-II • HDL subclasses • reverse cholesterol transport • cholesterol efflux

This article has been cited by other articles:

				D. C. Chan, G. F. Watts, E. M.M. Ooi, K.-A. Rye, J. Ji, A. G. Johnson, and P. H. R. Barrett Regulatory Effects of Fenofibrate and Atorvastatin on Lipoprotein A-I and Lipoprotein A-I:A-II Kinetics in the Metabolic Syndrome Diabetes Care, November 1, 2009; 32(11): 2111 - 2113. [Abstract] [Full Text] [PDF]

				E. M. M. Ooi, G. F. Watts, P. J. Nestel, D. Sviridov, A. Hoang, and P. H. R. Barrett Dose-Dependent Regulation of High-Density Lipoprotein Metabolism with Rosuvastatin in the Metabolic Syndrome J. Clin. Endocrinol. Metab., February 1, 2008; 93(2): 430 - 437. [Abstract] [Full Text] [PDF]

				J. Delgado-Lista, F. Perez-Jimenez, T. Tanaka, P. Perez-Martinez, Y. Jimenez-Gomez, C. Marin, J. Ruano, L. Parnell, J. M. Ordovas, and J. Lopez-Miranda An Apolipoprotein A-II Polymorphism (-265T/C, rs5082) Regulates Postprandial Response to a Saturated Fat Overload in Healthy Men J. Nutr., September 1, 2007; 137(9): 2024 - 2028. [Abstract] [Full Text] [PDF]

				J. Ji, G. F. Watts, A. G. Johnson, D. C. Chan, E. M. M. Ooi, K.-A. Rye, A. P. Serone, and P. H. R. Barrett High-Density Lipoprotein (HDL) Transport in the Metabolic Syndrome: Application of a New Model for HDL Particle Kinetics J. Clin. Endocrinol. Metab., March 1, 2006; 91(3): 973 - 979. [Abstract] [Full Text] [PDF]

				N. Fournier, A. Cogny, V. Atger, D. Pastier, D. Goudouneche, A. Nicoletti, N. Moatti, J. Chambaz, J.-L. Paul, and A.-D. Kalopissis Opposite Effects of Plasma From Human Apolipoprotein A-II Transgenic Mice on Cholesterol Efflux From J774 Macrophages and Fu5AH Hepatoma Cells Arterioscler Thromb Vasc Biol, April 1, 2002; 22(4): 638 - 643. [Abstract] [Full Text] [PDF]

				L. Saucan and E. A. Brinton Lp A-I and Niacin: New Views of an Antiatherogenic Duo Arterioscler Thromb Vasc Biol, November 1, 2001; 21(11): 1707 - 1709. [Full Text] [PDF]

				F. M. van 't Hooft, G. Ruotolo, S. Boquist, U. de Faire, G. Eggertsen, and A. Hamsten Human Evidence That the Apolipoprotein A-II Gene Is Implicated in Visceral Fat Accumulation and Metabolism of Triglyceride-Rich Lipoproteins Circulation, September 11, 2001; 104(11): 1223 - 1228. [Abstract] [Full Text] [PDF]

				A. Tailleux, M. Bouly, G. Luc, G. Castro, J.-M. Caillaud, N. Hennuyer, P. Poulain, J.-C. Fruchart, N. Duverger, and C. Fievet Decreased Susceptibility to Diet-Induced Atherosclerosis in Human Apolipoprotein A-II Transgenic Mice Arterioscler Thromb Vasc Biol, November 1, 2000; 20(11): 2453 - 2458. [Abstract] [Full Text] [PDF]

				M. Jauhiainen, J. Huuskonen, M. Baumann, J. Metso, T. Oka, T. Egashira, H. Hattori, V. M. Olkkonen, and C. Ehnholm Phospholipid transfer protein (PLTP) causes proteolytic cleavage of apolipoprotein A-I J. Lipid Res., April 1, 1999; 40(4): 654 - 664. [Abstract] [Full Text]

				M. Lee, A. von Eckardstein, L. Lindstedt, G. Assmann, and P. T. Kovanen Depletion of Preß1LpA1 and LpA4 Particles by Mast Cell Chymase Reduces Cholesterol Efflux From Macrophage Foam Cells Induced by Plasma Arterioscler Thromb Vasc Biol, April 1, 1999; 19(4): 1066 - 1074. [Abstract] [Full Text] [PDF]

				A. Ribeiro, D. Pastier, D. Kardassis, J. Chambaz, and P. Cardot Cooperative Binding of Upstream Stimulatory Factor and Hepatic Nuclear Factor 4 Drives the Transcription of the Human Apolipoprotein A-II Gene J. Biol. Chem., January 15, 1999; 274(3): 1216 - 1225. [Abstract] [Full Text] [PDF]

				P. J. Pussinen, M. Jauhiainen, J. Metso, L. E. Pyle, Y. L. Marcel, N. H. Fidge, and C. Ehnholm Binding of phospholipid transfer protein (PLTP) to apolipoproteins A-I and A-II: location of a PLTP binding domain in the amino terminal region of apoA-I J. Lipid Res., January 1, 1998; 39(1): 152 - 161. [Abstract] [Full Text]

				F. Blanco-Vaca, S.-J. Qu, C. Fiol, H.-Z. Fan, Q. Pao, A. Marzal-Casacuberta, J. J. Albers, I. Hurtado, V. Gracia, X. Pinto, et al. Molecular Basis of Fish-Eye Disease in a Patient From Spain : Characterization of a Novel Mutation in the LCAT Gene and Lipid Analysis of the Cornea Arterioscler Thromb Vasc Biol, July 1, 1997; 17(7): 1382 - 1391. [Abstract] [Full Text]

				W. Weng and J. L. Breslow Dramatically decreased high density lipoprotein cholesterol, increased remnant clearance, and insulin hypersensitivity in apolipoprotein A-II knockout mice suggest a complex role for apolipoprotein A-II in atherosclerosis susceptibility PNAS, December 10, 1996; 93(25): 14788 - 14794. [Abstract] [Full Text] [PDF]

				N. Fournier, A. Cogny, V. Atger, D. Pastier, D. Goudouneche, A. Nicoletti, N. Moatti, J. Chambaz, J.-L. Paul, and A.-D. Kalopissis Opposite Effects of Plasma From Human Apolipoprotein A-II Transgenic Mice on Cholesterol Efflux From J774 Macrophages and Fu5AH Hepatoma Cells Arterioscler Thromb Vasc Biol, April 1, 2002; 22(4): 638 - 643. [Abstract] [Full Text] [PDF]