© 1995 American Heart Association, Inc.
Articles |
Differentiated Macrophages Synthesize a Heparan Sulfate Proteoglycan and an Oversulfated Chondroitin Sulfate Proteoglycan That Bind Lipoprotein Lipase
From the Department of Comparative Medicine, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC (I.J.E., H.X., W.D.W.), and the Department of Medicine of the College of Physicians and Surgeons of Columbia University, New York, NY (J.C.O, I.J.G.).
Correspondence to Iris J. Edwards, PhD, Department of Comparative Medicine, Bowman Gray School of Medicine, Medical Center Blvd, Winston-Salem, NC 27157-1040.
Abstract Lipoprotein lipase (LpL), which facilitates lipoprotein uptake by macrophages, associates with the cell surface by binding to proteoglycans (PGs). Studies were designed to identify and characterize specific PGs that serve as receptors for LpL and to examine effects of cell differentiation on LpL binding. PG synthesis was examined by radiolabeling THP-1 monocytes and macrophages (a cell line originally derived from a patient with acute monocytic leukemia) with [35S]sodium sulfate and [3H]serine or [3H]glucosamine. Radiolabeled PGs isolated from the cell surface were purified by chromatography and identified as chondroitin-4–sulfate (CS) PG and heparan sulfate (HS) PG. A sixfold increase in CSPG and an 11-fold increase in HSPG accompanied cell differentiation. Whereas HS glycosaminoglycan chains from both monocytes and macrophages were 7.5 kD in size, CS chains increased in size from 17 kD to 36 kD with cell differentiation, and contained hexuronyl N-acetylgalactosamine-4,6-di-O sulfate disaccharides. LpL binding was sevenfold higher to differentiated cells, and affinity chromatography demonstrated that two cell surface PGs bound to LpL: HSPG and the oversulfated CSPG produced only by differentiated cells. We conclude that differentiation-associated changes in cell surface PG of human macrophages have functional consequences that could increase the atherogenic potential of the cells.
Key Words: macrophages • THP-1 cells • lipoprotein lipase • proteoglycans
This article has been cited by other articles:
 |
|
 |
|
  N. Malla, E. Berg, L. Uhlin-Hansen, and J.-O. Winberg Interaction of Pro-matrix Metalloproteinase-9/Proteoglycan Heteromer with Gelatin and Collagen J. Biol. Chem., May 16, 2008; 283(20): 13652 - 13665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. N. Birts, C. H. Barton, and D. C. Wilton A Catalytically Independent Physiological Function for Human Acute Phase Protein Group IIA Phospholipase A2: CELLULAR UPTAKE FACILITATES CELL DEBRIS REMOVAL J. Biol. Chem., February 22, 2008; 283(8): 5034 - 5045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Beeson, K. T. Andrews, M. Boyle, M. F. Duffy, E. K. Choong, T. J. Byrne, J. M. Chesson, A. M. Lawson, and W. Chai Structural Basis for Binding of Plasmodium falciparum Erythrocyte Membrane Protein 1 to Chondroitin Sulfate and Placental Tissue and the Influence of Protein Polymorphisms on Binding Specificity J. Biol. Chem., August 3, 2007; 282(31): 22426 - 22436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hashimura, K. Sudhir, J. Nigro, S. Ling, M. R. I. Williams, P. A. Komesaroff, and P. J. Little Androgens Stimulate Human Vascular Smooth Muscle Cell Proteoglycan Biosynthesis and Increase Lipoprotein Binding Endocrinology, April 1, 2005; 146(4): 2085 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Khalil, W. D. Wagner, and I. J. Goldberg Molecular Interactions Leading to Lipoprotein Retention and the Initiation of Atherosclerosis Arterioscler Thromb Vasc Biol, December 1, 2004; 24(12): 2211 - 2218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Gebska, I. Titley, H. F. Paterson, R. M. Morilla, D. C. Davies, A. M. Gruszka-Westwood, V. V. Kakkar, S. Eccles, and M. F. Scully High-affinity binding sites for heparin generated on leukocytes during apoptosis arise from nuclear structures segregated during cell death Blood, March 15, 2002; 99(6): 2221 - 2227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. O. Pentikainen, R. Oksjoki, K. Oorni, and P. T. Kovanen Lipoprotein Lipase in the Arterial Wall: Linking LDL to the Arterial Extracellular Matrix and Much More Arterioscler Thromb Vasc Biol, February 1, 2002; 22(2): 211 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ohtake, Y. Ito, M. Fukuta, and O. Habuchi Human N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase cDNA Is Related to Human B Cell Recombination Activating Gene-associated Gene J. Biol. Chem., November 16, 2001; 276(47): 43894 - 43900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kaplan and M. Aviram Retention of Oxidized LDL by Extracellular Matrix Proteoglycans Leads to Its Uptake by Macrophages : An Alternative Approach to Study Lipoproteins Cellular Uptake Arterioscler Thromb Vasc Biol, March 1, 2001; 21(3): 386 - 393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Mamputu, L. Levesque, and G. Renier Proliferative Effect of Lipoprotein Lipase on Human Vascular Smooth Muscle Cells Arterioscler Thromb Vasc Biol, October 1, 2000; 20(10): 2212 - 2219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Obunike, S. Pillarisetti, L. Paka, Y. Kako, M. J. Butteri, Y.-Y. Ho, W. D. Wagner, N. Yamada, T. Mazzone, R. J. Deckelbaum, et al. The Heparin-Binding Proteins Apolipoprotein E and Lipoprotein Lipase Enhance Cellular Proteoglycan Production Arterioscler Thromb Vasc Biol, January 1, 2000; 20(1): 111 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Clasper, S. Vekemans, M. Fiore, M. Plebanski, P. Wordsworth, G. David, and D. G. Jackson Inducible Expression of the Cell Surface Heparan Sulfate Proteoglycan Syndecan-2 (Fibroglycan) on Human Activated Macrophages Can Regulate Fibroblast Growth Factor Action J. Biol. Chem., August 20, 1999; 274(34): 24113 - 24123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Mamputu and G. Renier Differentiation of Human Monocytes to Monocyte-Derived Macrophages Is Associated With Increased Lipoprotein Lipase–Induced Tumor Necrosis Factor-{alpha} Expression and Production : A Process Involving Cell Surface Proteoglycans and Protein Kinase C Arterioscler Thromb Vasc Biol, June 1, 1999; 19(6): 1405 - 1411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Lundstam, E. Hurt-Camejo, G. Olsson, P. Sartipy, G. Camejo, and O. Wiklund Proteoglycans Contribution to Association of Lp(a) and LDL With Smooth Muscle Cell Extracellular Matrix Arterioscler Thromb Vasc Biol, May 1, 1999; 19(5): 1162 - 1167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. de Beer, W. L. Hendriks, L. C. van Vark, S. W.A. Kamerling, K. W. van Dijk, M. H. Hofker, A. H.M. Smelt, and L. M. Havekes Binding of ß-VLDL to Heparan Sulfate Proteoglycans Requires Lipoprotein Lipase, Whereas ApoE Only Modulates Binding Affinity Arterioscler Thromb Vasc Biol, March 1, 1999; 19(3): 633 - 637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Paka, Y. Kako, J. C. Obunike, and S. Pillarisetti Apolipoprotein E Containing High Density Lipoprotein Stimulates Endothelial Production of Heparan Sulfate Rich in Biologically Active Heparin-like Domains. A POTENTIAL MECHANISM FOR THE ANTI-ATHEROGENIC ACTIONS OF VASCULAR APOLIPOPROTEIN E J. Biol. Chem., February 19, 1999; 274(8): 4816 - 4823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Chang, K. L. Olin, C. Tsoi, T. N. Wight, and A. Chait Human Monocyte-derived Macrophages Secrete Two Forms of Proteoglycan-Macrophage Colony-stimulating Factor That Differ in Their Ability to Bind Low Density Lipoproteins J. Biol. Chem., June 26, 1998; 273(26): 15985 - 15992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kaplan, K. J. Williams, H. Mandel, and M. Aviram Role of Macrophage Glycosaminoglycans in the Cellular Catabolism of Oxidized LDL by Macrophages Arterioscler Thromb Vasc Biol, April 1, 1998; 18(4): 542 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-Y. Lin, M. Lucas, and T. Mazzone Endogenous apoE expression modulates HDL3 binding to macrophages J. Lipid Res., February 1, 1998; 39(2): 293 - 301. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Li, L. F. Brown, R. J. Laham, R. Volk, and M. Simons Macrophage-Dependent Regulation of Syndecan Gene Expression Circ. Res., November 19, 1997; 81(5): 785 - 796. [Abstract] [Full Text]
|
|
|
|
|
|
A. Kinoshita, S. Yamada, S. M. Haslam, H. R. Morris, A. Dell, and K. Sugahara Novel Tetrasaccharides Isolated from Squid Cartilage Chondroitin Sulfate E Contain Unusual Sulfated Disaccharide Units GlcA(3-O-sulfate)beta 1-3GalNAc(6-O-sulfate) or GlcA(3-O-sulfate)beta 1-3GalNAc(4,6-O-disulfate) J. Biol. Chem., August 8, 1997; 272(32): 19656 - 19665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hurt-Camejo, U. Olsson, O. Wiklund, G. Bondjers, and G. Camejo Cellular Consequences of the Association of ApoB Lipoproteins With Proteoglycans: Potential Contribution to Atherogenesis Arterioscler Thromb Vasc Biol, June 1, 1997; 17(6): 1011 - 1017. [Abstract] [Full Text]
|
|
|
|
|
|
M. Lucas and T. Mazzone Cell Surface Proteoglycans Modulate Net Synthesis and Secretion of Macrophage Apolipoprotein E J. Biol. Chem., June 7, 1996; 271(23): 13454 - 13460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ueno, S. Yamada, M. Zako, M. Bernfield, and K. Sugahara Structural Characterization of Heparan Sulfate and Chondroitin Sulfate of Syndecan-1 Purified from Normal Murine Mammary Gland Epithelial Cells. COMMON PHOSPHORYLATION OF XYLOSE AND DIFFERENTIAL SULFATION OF GALACTOSE IN THE PROTEIN LINKAGE REGION TETRASACCHARIDE SEQUENCE J. Biol. Chem., July 27, 2001; 276(31): 29134 - 29140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ito and O. Habuchi Purification and Characterization of N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase from the Squid Cartilage J. Biol. Chem., October 27, 2000; 275(44): 34728 - 34736. [Abstract] [Full Text] [PDF]
|
|