© 1995 American Heart Association, Inc.
Articles |
Quantification of Postprandial Triglyceride-Rich Lipoproteins in Healthy Men by Retinyl Ester Labeling and Simultaneous Measurement of Apolipoproteins B-48 and B-100
From the Atherosclerosis Research Unit, King Gustaf V Research Institute, Department of Medicine, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
Correspondence to Fredrik Karpe, Atherosclerosis Research Unit, King Gustaf V Research Institute, Department of Medicine, Karolinska Hospital, Karolinska Institute, S-171 76, Stockholm, Sweden.
Abstract The metabolism of chylomicrons, very-low-density lipoprotein (VLDL), and their remnants in the postprandial state was studied in normolipidemic healthy men by measuring apoB-48 and apoB-100 and retinyl palmitate (RP) in fractions of triglyceride-rich lipoproteins after a mixed meal type of oral fat load supplemented with vitamin A. ApoB-48 was present at low concentrations in the fasting plasma samples in most subjects and increased in response to the test meal in Svedberg's flotation rate (Sf) >20 lipoprotein fractions. Concomitantly, the level of Sf 60 to 400 apoB-100 (large VLDL) had doubled at 3 hours and returned to baseline at 9 hours. The number of apoB-48–containing lipoprotein particles did not exceed 20% of the total number of apoB-containing lipoproteins contained in Sf 12 to 400 fractions at any time point after fat intake. The peak plasma level of RP was delayed compared with the peak plasma concentration of apoB-48, suggesting that retinyl ester labeling of chylomicrons is questionable as a means of quantifying postprandial triglyceride-rich lipoproteins of intestinal origin. Approximately 2000 and 4000 RP molecules were carried in each chylomicron particle in the 3- and 6-hour samples, respectively, in contrast to the remnant fractions in which 100 to 600 RP molecules were found for each lipoprotein particle. The limited RP exchange between lipoprotein particles indicates that the smaller intestinal lipoproteins do no originate primarily from larger Sf >400 chylomicron particles but instead are secreted directly into the Sf 20 to 400 fraction and subsequently converted to smaller chylomicron remnants. This notion was further substantiated by injection of RP-labeled postprandial plasma into fasting subjects with subsequent tracing of RP in fractions of triglyceride-rich lipoproteins.
Key Words: intestinal lipoproteins • chylomicron remnants • lipolysis • plasma triglycerides • vitamin A
This article has been cited by other articles:
 |
|
 |
|
  M. Adiels, S.-O. Olofsson, M.-R. Taskinen, and J. Boren Overproduction of Very Low-Density Lipoproteins Is the Hallmark of the Dyslipidemia in the Metabolic Syndrome Arterioscler. Thromb. Vasc. Biol., July 1, 2008; 28(7): 1225 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. D. Knuth and J. F. Horowitz The Elevation of Ingested Lipids within Plasma Chylomicrons Is Prolonged in Men Compared with Women J. Nutr., June 1, 2006; 136(6): 1498 - 1503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Verseyden, S. Meijssen, H. van Dijk, H. Jansen, and M. C. Cabezas Effects of atorvastatin on fasting and postprandial complement component 3 response in familial combined hyperlipidemia J. Lipid Res., November 1, 2003; 44(11): 2100 - 2108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Mattes Oral Fat Exposure Increases the First Phase Triacylglycerol Concentration Due to Release of Stored Lipid in Humans J. Nutr., December 1, 2002; 132(12): 3656 - 3662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G Jackson, M D. Robertson, B. A Fielding, K. N Frayn, and C. M Williams Olive oil increases the number of triacylglycerol-rich chylomicron particles compared with other oils: an effect retained when a second standard meal is fed Am. J. Clinical Nutrition, November 1, 2002; 76(5): 942 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bjorkegren, A. Silveira, S. Boquist, R. Tang, F. Karpe, M. G. Bond, U. de Faire, and A. Hamsten Postprandial Enrichment of Remnant Lipoproteins With ApoC-I in Healthy Normolipidemic Men With Early Asymptomatic Atherosclerosis Arterioscler. Thromb. Vasc. Biol., September 1, 2002; 22(9): 1470 - 1474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Couillard, N. Bergeron, A. Pascot, N. Almeras, J. Bergeron, A. Tremblay, D. Prud'homme, and J.-P. Despres Evidence for impaired lipolysis in abdominally obese men: postprandial study of apolipoprotein B-48- and B-100-containing lipoproteins Am. J. Clinical Nutrition, August 1, 2002; 76(2): 311 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kovar and R. J. Havel Sources and properties of triglyceride-rich lipoproteins containing apoB-48 and apoB-100 in postprandial blood plasma of patients with primary combined hyperlipidemia J. Lipid Res., July 1, 2002; 43(7): 1026 - 1034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Verseyden, S. Meijssen, and M. C. Cabezas Postprandial changes of apoB-100 and apoB-48 in TG rich lipoproteins in familial combined hyperlipidemia J. Lipid Res., February 1, 2002; 43(2): 274 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhou and A. Nilsson Sources of eicosanoid precursor fatty acid pools in tissues J. Lipid Res., October 1, 2001; 42(10): 1521 - 1542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Ooi, M. Cousins, D. S. Ooi, G. Steiner, K. D. Uffelman, K. Nakajima, and I. E. Simo Postprandial Remnant-like Lipoproteins in Hypertriglyceridemia J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3134 - 3142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ylitalo, P. Pajukanta, S. Meri, R. M. Cantor, N. Mero-Matikainen, J. Vakkilainen, I. Nuotio, and M.-R. Taskinen Serum C3 but Not Plasma Acylation-Stimulating Protein Is Elevated in Finnish Patients With Familial Combined Hyperlipidemia Arterioscler. Thromb. Vasc. Biol., May 1, 2001; 21(5): 838 - 843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tholstrup, B. Sandstrom, A. Bysted, and G. Holmer Effect of 6 dietary fatty acids on the postprandial lipid profile, plasma fatty acids, lipoprotein lipase, and cholesterol ester transfer activities in healthy young men Am. J. Clinical Nutrition, February 1, 2001; 73(2): 198 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Parhofer, P. H. R. Barrett, and P. Schwandt Atorvastatin Improves Postprandial Lipoprotein Metabolism in Normolipidemic Subjects J. Clin. Endocrinol. Metab., November 1, 2000; 85(11): 4224 - 4230. [Abstract] [Full Text]
|
|
|
|
|
|
X. Hu, R. J Jandacek, and W. S White Intestinal absorption of {beta}-carotene ingested with a meal rich in sunflower oil or beef tallow: postprandial appearance in triacylglycerol-rich lipoproteins in women Am. J. Clinical Nutrition, May 1, 2000; 71(5): 1170 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Cohn, C. Marcoux, and J. Davignon Detection, Quantification, and Characterization of Potentially Atherogenic Triglyceride-Rich Remnant Lipoproteins Arterioscler. Thromb. Vasc. Biol., October 1, 1999; 19(10): 2474 - 2486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-Q. Xiang, K. Cianflone, D. Kalant, and A. D. Sniderman Differential binding of triglyceride-rich lipoproteins to lipoprotein lipase J. Lipid Res., September 1, 1999; 40(9): 1655 - 1663. [Abstract] [Full Text]
|
|
|
|
|
|
N. Mero, L. Suurinkeroinen, M. Syvänne, P. Knudsen, H. Yki-Järvinen, and M.-R. Taskinen Delayed clearance of postprandial large TG-rich particles in normolipidemic carriers of LPL Asn291Ser gene variant J. Lipid Res., September 1, 1999; 40(9): 1663 - 1670. [Abstract] [Full Text]
|
|
|
|
|
|
S. Hadjadj, J.-L. Paul, L. Meyer, V. Durlach, B. Vergès, O. Ziegler, P. Drouin, and B. Guerci Delayed Changes in Postprandial Lipid in Young Normolipidemic Men after a Nocturnal Vitamin A Oral Fat Load Test J. Nutr., September 1, 1999; 129(9): 1649 - 1655. [Abstract] [Full Text]
|
|
|
|
|
|
C. Thomsen, O. Rasmussen, T. Lousen, J. J Holst, S. Fenselau, J. Schrezenmeir, and K. Hermansen Differential effects of saturated and monounsaturated fatty acids on postprandial lipemia and incretin responses in healthy subjects Am. J. Clinical Nutrition, June 1, 1999; 69(6): 1135 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. F. Tinker, E. J Parks, S. R. Behr, B. O. Schneeman, and P. A. Davis (n-3) Fatty Acid Supplementation in Moderately Hypertriglyceridemic Adults Changes Postprandial Lipid and Apolipoprotein B Responses to a Standardized Test Meal J. Nutr., June 1, 1999; 129(6): 1126 - 1134. [Abstract] [Full Text]
|
|
|
|
|
|
S. Lemieux, R. Fontani, K. D. Uffelman, G. F. Lewis, and G. Steiner Apolipoprotein B-48 and retinyl palmitate are not equivalent markers of postprandial intestinal lipoproteins J. Lipid Res., October 1, 1998; 39(10): 1964 - 1971. [Abstract] [Full Text]
|
|
|
|
|
|
H. Tong, H. R. Knapp, and M. VanRollins A low temperature flotation method to rapidly isolate lipoproteins from plasma J. Lipid Res., August 1, 1998; 39(8): 1696 - 1704. [Abstract] [Full Text]
|
|
|
|
|
|
D. S. Freedman, J. D. Otvos, E. J. Jeyarajah, J. J. Barboriak, A. J. Anderson, and J. A. Walker Relation of Lipoprotein Subclasses as Measured by Proton Nuclear Magnetic Resonance Spectroscopy to Coronary Artery Disease Arterioscler. Thromb. Vasc. Biol., July 1, 1998; 18(7): 1046 - 1053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Talmud, S. Hall, S. Holleran, R. Ramakrishnan, H. N. Ginsberg, and S. E. Humphries LPL promoter -93T/G transition influences fasting and postprandial plasma triglycerides response in African-Americans and Hispanics J. Lipid Res., June 1, 1998; 39(6): 1189 - 1196. [Abstract] [Full Text]
|
|
|
|
|
|
A. P. van Beek, H. H. J. J. van Barlingen, F. C. de Ruijter-Heijstek, H. Jansen, D. W. Erkelens, G. M. Dallinga-Thie, and T. W. A. de Bruin Preferential clearance of apoB-48-containing lipoproteins after heparin-induced lipolysis is modulated by lipoprotein lipase activity J. Lipid Res., February 1, 1998; 39(2): 322 - 332. [Abstract] [Full Text]
|
|
|
|
|
|
N. Mero, M. Syvanne, B. Eliasson, U. Smith, and M.-R. Taskinen Postprandial Elevation of ApoB-48-Containing Triglyceride-Rich Particles and Retinyl Esters in Normolipemic Males Who Smoke Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 2096 - 2102. [Abstract] [Full Text]
|
|
|
|
|
|
A. Silveira, F. Karpe, H. Johnsson, K. A. Bauer, and A. Hamsten In Vivo Demonstration in Humans That Large Postprandial Triglyceride-Rich Lipoproteins Activate Coagulation Factor VII Through the Intrinsic Coagulation Pathway Arterioscler. Thromb. Vasc. Biol., November 1, 1996; 16(11): 1333 - 1339. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. S Weintraub, I. Grosskopf, T. Rassin, H. Miller, G. Charach, H. H Rotmensch, M. Liron, A. Rubinstein, and A. Iaina Clearance of chylomicron remnants in normolipidaemic patients with coronary artery disease: case control study over three years BMJ, April 13, 1996; 312(7036): 935 - 939. [Abstract] [Full Text]
|
|