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(Arteriosclerosis, Thrombosis, and Vascular Biology. 1995;15:1875-1881.)
© 1995 American Heart Association, Inc.

Articles

Lipid Composition of HDL Subfractions in Dog Plasma and Lymph

Laurence Wong; Bela Sivok; Eva Kurucz; Charles H. Sloop; Paul S. Roheim; Bela Asztalos

From the Division of Lipoprotein Metabolism and Pathophysiology, Department of Physiology, Louisiana State University Medical Center, New Orleans.

Correspondence to Laurence Wong, Division of Lipoprotein Metabolism and Pathophysiology, Department of Physiology, Louisiana State University Medical Center, New Orleans, LA 70112.

Abstract We report the lipid composition of dog plasma and peripheral lymph lipoproteins as separated into pre-ß, , and pre- fractions by agarose gel electrophoresis. Plasma lipoproteins with mobility have a composition different from that of plasma lipoproteins with pre- mobility, having 9% versus 11% free cholesterol, 21% versus 17% cholesterol ester, 1% versus 16% triacylglycerol, and 69% versus 56% phospholipid. On the other hand, lymph and pre- lipoproteins have compositions that are quite similar (9% versus 7% free cholesterol, 17% versus 17% cholesterol ester, 2% versus 4% triacylglycerol, and 71% versus 71% phospholipid). The lipid compositions of plasma and lymph lipoproteins are quite similar (9% versus 9% free cholesterol, 21% versus 17% cholesterol ester, 1% versus 2% triacylglycerol, and 70% versus 72% phospholipid). The lipid compositions of plasma and lymph pre- lipoproteins are different (11% versus 7% free cholesterol, 17% versus 17% cholesterol ester, 16% versus 4% triacylglycerol, and 56% versus 71% phospholipid). Peripheral lymph lipoproteins with pre-ß mobility contained 15% cholesterol, 13% cholesterol ester, 10% triacylglycerol, and 61% phospholipid. Compared with plasma, peripheral lymph lipoproteins are free cholesterol–enriched in all fractions. Calculated stoichiometric ratios of lipid to apoA-I indicate that pre-ß lipoproteins contain one molecule of apoA-I per particle, lipoproteins have two molecules of apoA-I per particle, and pre- lipoproteins have four molecules of apoA-I per particle.

Key Words: high density lipoproteins • phospholipids • cholesterol • cholesterol ester • triacylglycerol

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