Arteriosclerosis and Thrombosis, Vol 14, 727-733, Copyright © 1994 by American Heart Association

ARTICLES

Cell proliferation after balloon injury of iliac arteries in the cholesterol-fed New Zealand White rabbit

ML Stadius, AM Gown, R Kernoff and CL Collins
Department of Medicine, University of Washington, Seattle.

Acute mechanical injury of an artery results in neointimal hyperplasia that is due at least in part to cell proliferation within the vessel wall. The purpose of this study was to quantify cell proliferation activity in the iliac artery of New Zealand White rabbits after balloon injury and cholesterol feeding. Retrograde pullback balloon injury of iliac arteries was performed, and the animals were then fed a 2% cholesterol diet. At intervals from day 1 through day 35 postinjury, iliac arteries were obtained for histological analysis. Intimal and medial areas were measured morphometrically. Total number of cells within the intima and media was counted. Smooth muscle cell-predominant or macrophage-predominant regions of the intima and media were identified using HHF-35 and RAM-11 immunocytochemical markers, respectively. Number of cells in the proliferative phase of the cell cycle was measured by using the proliferating cell nuclear antigen and bromodeoxyuridine techniques. Thirty-one arteries from 16 rabbits were available for analysis. Total number of cells and number of cells per square millimeter within the media did not change significantly from day 1 through day 35 postinjury. Total number of cells within the intima increased significantly, but the number of cells per square millimeter of intima decreased significantly during the same time period. Proliferative activity was identified in the media between days 3 and 35 with peak activity at day 3 postinjury. Proliferative activity in the intima was present in all specimens from day 8 through day 35. Proliferative activity was present in both HHF-35- and RAM-11- predominant regions of the intima.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				A. G. Touchard and R. S. Schwartz Preclinical Restenosis Models: Challenges and Successes Toxicol Pathol, January 1, 2006; 34(1): 11 - 18. [Abstract] [Full Text] [PDF]

				M. B. Kahn, K. Boesze-Battaglia, D. W. Stepp, A. Petrov, Y. Huang, R. P. Mason, and T. N. Tulenko Influence of serum cholesterol on atherogenesis and intimal hyperplasia after angioplasty: inhibition by amlodipine Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H591 - H600. [Abstract] [Full Text] [PDF]

				N. M. Rajamannan, M. Subramaniam, M. Springett, T. C. Sebo, M. Niekrasz, J. P. McConnell, R. J. Singh, N. J. Stone, R. O. Bonow, and T. C. Spelsberg Atorvastatin Inhibits Hypercholesterolemia-Induced Cellular Proliferation and Bone Matrix Production in the Rabbit Aortic Valve Circulation, June 4, 2002; 105(22): 2660 - 2665. [Abstract] [Full Text] [PDF]

				H. O. Andersen, B. F. Hansen, P. Holm, S. Stender, and B. G. Nordestgaard Effect of Cyclosporine on Arterial Balloon Injury Lesions in Cholesterol-Clamped Rabbits : T Lymphocyte–Mediated Immune Responses Not Involved in Balloon Injury–Induced Neointimal Proliferation Arterioscler Thromb Vasc Biol, July 1, 1999; 19(7): 1687 - 1694. [Abstract] [Full Text] [PDF]

				M. Takagi, M. Ueda, A. E. Becker, K. Takeuchi, and T. Takeda The Watanabe Heritable Hyperlipidemic Rabbit Is a Suitable Experimental Model to Study Differences in Tissue Response Between Intimal and Medial Injury After Balloon Angioplasty Arterioscler Thromb Vasc Biol, December 1, 1997; 17(12): 3611 - 3619. [Abstract] [Full Text]

				S.D. Gertz, W. L Barry, L. W Gimple, Shmuel Banai, L. S Perez, C. A McNamara, E. R Powers, M. Ragosta, G. K Owens, W. C Roberts, et al. Predictors of luminal narrowing by neointima after angioplasty in atherosclerotic rabbits Cardiovasc Res, December 1, 1997; 36(3): 396 - 407. [Abstract] [Full Text] [PDF]

				C. R. White, J. Shelton, S.-J. Chen, V. Darley-Usmar, L. Allen, C. Nabors, P. W. Sanders, Y.-F. Chen, and S. Oparil Estrogen Restores Endothelial Cell Function in an Experimental Model of Vascular Injury Circulation, September 2, 1997; 96(5): 1624 - 1630. [Abstract] [Full Text]

				P. G. Steg, M. Ziol, O. Tahlil, C. Robert, P. Masson, D. Pruneau, P. Bruneval, and P. Belichard Reduction of Intimal Hyperplasia by Naroparcil, a 4-Methylumbelliferyl ß-D-Xyloside Analogue, After Arterial Injury in the Hypercholesterolemic Rabbit Circ. Res., November 1, 1995; 77(5): 919 - 926. [Abstract] [Full Text]