Donate Help Contact The AHA Sign In Home
American Heart Association
Arteriosclerosis, Thrombosis, and Vascular Biology
Search: search_blue_button Advanced Search
« Previous Article | Table of Contents | Next Article »
Arteriosclerosis, Thrombosis, and Vascular Biology. 1991;11:851-856

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tenkanen, H.
Right arrow Articles by Ehnholm, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tenkanen, H.
Right arrow Articles by Ehnholm, C.

Arteriosclerosis and Thrombosis, Vol 11, 851-856, Copyright © 1991 by American Heart Association

ARTICLES

The mutation causing the common apolipoprotein A-IV polymorphism is a glutamine to histidine substitution of amino acid 360

H Tenkanen, M Lukka, M Jauhiainen, J Metso, M Baumann, L Peltonen and C Ehnholm
National Public Health Institute, Helsinki, Finland.

Apolipoprotein (apo) A-IV is a protein involved in the metabolism of chylomicrons and high density lipoproteins. This protein displays genetic polymorphism due to two main codominant alleles, A-IV1 and A- IV2. We have identified the mutation that leads to this polymorphism. It is caused by a single-base substitution of guanine for thymine in the third base of codon 360. This substitution leads to a glutamine to histidine change. Direct sequencing of amplified DNA from eight subjects in a three-generation pedigree has demonstrated that the guanine to thymine substitution can explain the apo A-IV polymorphism. In 32 unrelated individuals, a correspondence between apo A-IV phenotype determined by isoelectric focusing and genotype determined with Fnu4HI digestion of amplified DNA could be demonstrated. The enzyme lecithin:cholesteryl acyltransferase (LCAT) is activated by apo A-IV. Under our in vitro conditions, the isoprotein apo A-IV 1-1 is a better LCAT activator than is the isoprotein apo A-IV 2-2. A knowledge of the molecular mechanism underlying the apo A-IV polymorphism will help to elucidate the mechanisms involved in LCAT activation.


This article has been cited by other articles:


Home page
 Int J EpidemiolHome page
M. A Pereira, R. M Weggemans, D. R Jacobs Jr, P. J Hannan, P. L Zock, J. M Ordovas, and M. B Katan
Within-person variation in serum lipids: implications for clinical trials
Int. J. Epidemiol., June 1, 2004; 33(3): 534 - 541.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
M. Kratz, U. Wahrburg, A. von Eckardstein, B. Ezeh, G. Assmann, and F. Kronenberg
Dietary Mono- and Polyunsaturated Fatty Acids Similarly Increase Plasma Apolipoprotein A-IV Concentrations in Healthy Men and Women
J. Nutr., June 1, 2003; 133(6): 1821 - 1825.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
R. M. Weggemans, P. L. Zock, S. Meyboom, H. Funke, and M. B. Katan
Apolipoprotein A4-1/2 polymorphism and response of serum lipids to dietary cholesterol in humans
J. Lipid Res., October 1, 2000; 41(10): 1623 - 1628.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
H. Campos, J. Lopez-Miranda, C. Rodriguez, M. Albajar, E. J. Schaefer, and J. M. Ordovas
Urbanization Elicits a More Atherogenic Lipoprotein Profile in Carriers of the Apolipoprotein A-IV-2 Allele Than in A-IV-1 Homozygotes
Arterioscler Thromb Vasc Biol, June 1, 1997; 17(6): 1074 - 1081.
[Abstract] [Full Text]


ATVB Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 1991 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.