Arteriosclerosis, Vol 10, 559-563, Copyright © 1990 by American Heart Association
ARTICLES |
Platelet secretory products increase low density lipoprotein oxidation, enhance its uptake by macrophages, and reduce its fluidity
M Aviram, G Dankner and JG Brook
Lipid Research Laboratory, Rambam Medical Center, Haifa, Israel.
Oxidized low density lipoprotein (Ox-LDL) is considered to be involved in the atherogenic process. Factors influencing the formation of Ox-LDL are thus of importance. Oxidation of LDL in a cell-free system in the presence of copper ions was significantly increased (up to 60%) by the presence of platelet-conditioned medium, (PCM) obtained from collagen- activated platelets for the duration of the oxidation period. The effect was time- and dose-dependent and was related to hydrogen peroxide and superoxide production, since PCM-induced enhanced LDL oxidation was inhibited by catalase and by superoxide dismutase, but not by protease treatments. PCM also reduced the fluidity of oxidized LDL by 45%. Upon incubation with a J-774 macrophage-like cell line, PCM- treated Ox-LDL enhanced cellular cholesteryl ester synthesis by 47% and lipoprotein degradation by 41%. Thus platelet secretory products appeared to enhance LDL oxidation through the involvement of oxidative agents. The resulting Ox-LDL demonstrated increased atherogenic properties.
This article has been cited by other articles:
 |
|
 |
|
  L. G. Coleman Jr, R. K. Polanowska-Grabowska, M. Marcinkiewicz, and A. R. L. Gear LDL oxidized by hypochlorous acid causes irreversible platelet aggregation when combined with low levels of ADP, thrombin, epinephrine, or macrophage-derived chemokine (CCL22) Blood, July 15, 2004; 104(2): 380 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kaplan, T. Hayek, A. Raz, R. Coleman, L. Dornfeld, J. Vaya, and M. Aviram Pomegranate Juice Supplementation to Atherosclerotic Mice Reduces Macrophage Lipid Peroxidation, Cellular Cholesterol Accumulation and Development of Atherosclerosis J. Nutr., August 1, 2001; 131(8): 2082 - 2089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kaplan and M. Aviram Retention of Oxidized LDL by Extracellular Matrix Proteoglycans Leads to Its Uptake by Macrophages : An Alternative Approach to Study Lipoproteins Cellular Uptake Arterioscler. Thromb. Vasc. Biol., March 1, 2001; 21(3): 386 - 393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Volf, E. Bielek, T. Moeslinger, F. Koller, and E. Koller Modification of Protein Moiety of Human Low Density Lipoprotein by Hypochlorite Generates Strong Platelet Agonist Arterioscler. Thromb. Vasc. Biol., August 1, 2000; 20(8): 2011 - 2018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Corsini Reviews: Fluvastatin: Effects Beyond Cholesterol Lowering Journal of Cardiovascular Pharmacology and Therapeutics, January 1, 2000; 5(3): 161 - 175. [Abstract] [PDF]
|
|
|
|
|
|
M. Kaplan, K. J. Williams, H. Mandel, and M. Aviram Role of Macrophage Glycosaminoglycans in the Cellular Catabolism of Oxidized LDL by Macrophages Arterioscler. Thromb. Vasc. Biol., April 1, 1998; 18(4): 542 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Hsu, A. C. Nicholson, and D. P. Hajjar Inhibition of Macrophage Scavenger Receptor Activity by Tumor Necrosis Factor-alpha Is Transcriptionally and Post-transcriptionally Regulated J. Biol. Chem., March 29, 1996; 271(13): 7767 - 7773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Keidar, M. Kaplan, and M. Aviram Angiotensin II–Modified LDL Is Taken Up by Macrophages Via the Scavenger Receptor, Leading to Cellular Cholesterol Accumulation Arterioscler. Thromb. Vasc. Biol., January 1, 1996; 16(1): 97 - 105. [Abstract] [Full Text]
|
|