Arteriosclerosis, Vol 1, 177-185, Copyright © 1981 by American Heart Association

ARTICLES

Acetoacetylated lipoproteins used to distinguish fibroblasts from macrophages in vitro by fluorescence microscopy

RE Pitas, TL Innerarity, JN Weinstein and RW Mahley

We have developed a procedure for labeling lipoproteins with the fluorescent probe 3,3'-dioctadecylindocarbocyanine (Dil) and have used Dil-labeled native and acetoacetylated lipoproteins to differentiate macrophages from fibroblasts in mixed cell culture. Lipoproteins labeled with this probe were suitable for the direct viewing of their binding and internalization by cells in vitro. The labeling technique has been applied to human low density lipoproteins (LDL) and to two canine cholesterol-induced lipoproteins: apo-E HDLc, which contain only the E apoprotein (apo-E), and beta-migrating, very low density lipoproteins (beta-VLDL), which contain apo-B and apo-E. The Dil- labeled lipoproteins showed specific high affinity binding to human fibroblasts via the LDL (apo-B, -E) receptors. The equilibrium dissociation constant for the binding of Dil-labeled apo-E HDLc and LDL were the same as for the respective native lipoproteins. The specific binding of Dil-labeled LDL and apo-E HDLc was further substantiated by fluorescence microscopy. When an excess of native (non-fluorescent) lipoproteins was added to the Dil-labeled lipoproteins, essentially no fluorescently labeled lipoproteins were seen associated with the cells. The Dil-labeled LDL, apo-E HDLc, and beta-VLDL, which were bound to the cells at 4 degrees C, were associated with the cell surface and were often observed in linear arrays. Cells that were either incubated with Dil-labeled lipoproteins at 4 degrees C and subsequently heated to 37 degrees C or incubated with the Dil-labeled lipoproteins at 37 degrees C showed internalized perinuclear fluorescence. When Dil-labeled LDL, apo-E HDLc, or beta-VLDL were treated with diketene to acetoacetylate their lysine residues, and then were incubated at 37 degrees C with mixtures of fibroblasts and mouse peritoneal macrophages in culture, the fibroblasts did not become fluorescently labeled. The macrophages became highly fluorescent, however. The acetoacetylation inhibited the interaction of the lipoproteins with the apo-B, -E receptors of fibroblasts and stimulated their uptake by macrophages. The use of fluorescently labeled native lipoproteins and chemically modified lipoproteins may allow the functional differentiation of macrophages from other cell types (e.g., fibroblasts and smooth muscle cells) in the arterial wall. This differentiation may be useful in determining the origin of the lipid-laden foam cells of atherosclerotic lesions.

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				K. L. Wyne, R. K. Pathak, M. C. Seabra, and H. H. Hobbs Expression of the VLDL Receptor in Endothelial Cells Arterioscler Thromb Vasc Biol, March 1, 1996; 16(3): 407 - 415. [Abstract] [Full Text]

				S. Bellosta, B. P. Nathan, M. Orth, L.-M. Dong, R. W. Mahley, and R. E. Pitas Stable Expression and Secretion of Apolipoproteins E3 and E4 in Mouse Neuroblastoma Cells Produces Differential Effects on Neurite Outgrowth J. Biol. Chem., November 10, 1995; 270(45): 27063 - 27071. [Abstract] [Full Text] [PDF]

				Q. Gong and R. E. Pitas Synergistic Effects of Growth Factors on the Regulation of Smooth Muscle Cell Scavenger Receptor Activity J. Biol. Chem., September 15, 1995; 270(37): 21672 - 21678. [Abstract] [Full Text] [PDF]

				F. Bernini, N. Scurati, G. Bonfadini, and R. Fumagalli HMG-CoA Reductase Inhibitors Reduce Acetyl LDL Endocytosis in Mouse Peritoneal Macrophages Arterioscler Thromb Vasc Biol, September 1, 1995; 15(9): 1352 - 1358. [Abstract] [Full Text]

				T. M. Stulnig, H. Klocker, H. J. Harwood Jr, G. Jurgens, D. Schonitzer, E. Jarosch, L. A. Huber, A. Amberger, and G. Wick In Vivo LDL Receptor and HMG-CoA Reductase Regulation in Human Lymphocytes and Its Alterations During Aging Arterioscler Thromb Vasc Biol, July 1, 1995; 15(7): 872 - 878. [Abstract] [Full Text]

				J. A. Kim, J.-L. Gu, R. Natarajan, J. A. Berliner, and J. L. Nadler A Leukocyte Type of 12-Lipoxygenase Is Expressed in Human Vascular and Mononuclear Cells : Evidence for Upregulation by Angiotensin II Arterioscler Thromb Vasc Biol, July 1, 1995; 15(7): 942 - 948. [Abstract] [Full Text]

				S. Takahashi, J. Suzuki, M. Kohno, K. Oida, T. Tamai, S. Miyabo, T. Yamamoto, and T. Nakai Enhancement of the Binding of Triglyceride-rich Lipoproteins to the Very Low Density Lipoprotein Receptor by Apolipoprotein E and Lipoprotein Lipase J. Biol. Chem., June 30, 1995; 270(26): 15747 - 15754. [Abstract] [Full Text] [PDF]

				M. Rommeswinkel, N. J. Severs, M. Koster, and H. Robenek Repression of the Macrophage Scavenger Receptor in Macrophage–Smooth Muscle Cell Heterokaryons Arterioscler Thromb Vasc Biol, May 1, 1995; 15(5): 601 - 611. [Abstract] [Full Text]

				R. Ghosh, D. Gelman, and F. Maxfield Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy J. Cell Sci., January 8, 1994; 107(8): 2177 - 2189. [Abstract] [PDF]

				B. Watkins, H. Dorn, W. Kelly, R. Armstrong, B. Potts, F Michaels, C. Kufta, and M Dubois-Dalcq Specific tropism of HIV-1 for microglial cells in primary human brain cultures Science, August 3, 1990; 249(4968): 549 - 553. [Abstract] [PDF]

				E. Nabel, G Plautz, F. Boyce, J. Stanley, and G. Nabel Recombinant gene expression in vivo within endothelial cells of the arterial wall Science, June 16, 1989; 244(4910): 1342 - 1344. [Abstract] [PDF]

				S. Hofmann, D. Russell, M. Brown, J. Goldstein, and R. Hammer Overexpression of low density lipoprotein (LDL) receptor eliminates LDL from plasma in transgenic mice Science, March 11, 1988; 239(4845): 1277 - 1281. [Abstract] [PDF]

				M. Ignatius, E. Shooter, R. Pitas, and R. Mahley Lipoprotein uptake by neuronal growth cones in vitro Science, May 22, 1987; 236(4804): 959 - 962. [Abstract] [PDF]

				L Hobbie, D. Kingsley, K. Kozarsky, R. Jackman, and M Krieger Restoration of LDL receptor activity in mutant cells by intercellular junctional communication Science, January 2, 1987; 235(4784): 69 - 73. [Abstract] [PDF]

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