on October 17, 2002
Published online before print October 17, 2002, doi: 10.1161/01.ATV.0000042206.98651.15
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 29, 2002
Accepted on September 30, 2002
Differential Gene Expression in Vascular Smooth Muscle Cells in Primary Atherosclerosis and In Stent Stenosis in Humans
Qi Jun Zhang ;
From the Division of Cardiovascular Medicine (Q.J.Z., C.S., M.B.), Addenbrooke's Centre for Clinical Investigation, Addenbrooke's Hospital and Department of Histopathology (M.G.) and Cardiac Unit (L.S.), Papworth Hospital, Cambridge, UK.
* To whom correspondence should be addressed. E-mail: mrb{at}mole.bio.cam.ac.uk.
Objective—We sought to identify differentially expressed genes in human in stent stenosis (ISS) to provide insights into the mechanism of disease.
Methods and Results—Using representation difference analysis, we examined differential gene expression between cultured normal human medial vascular smooth muscle cells (VSMCs) and cells from primary atherosclerotic plaques or ISS sites. Specific groups of genes were overexpressed in ISS and plaque VSMCs, including cell cycle regulatory proteins and cell matrix and contractile proteins. Differential expression was validated by virtual Northern analysis, reverse transcriptase-polymerase chain reaction, in situ hybridization, and immunohistochemistry. All ISS genes were expressed by normal intima and had even higher expression in primary plaque VSMCs.
Conclusions—ISS VSMCs have a stable gene expression profile reflecting an intimal pattern, intermediate between normal medial and primary plaque VSMCs. Differential expression profiling may identify markers of disease that are overexpressed in ISS and also help elucidate the origin of the ISS lesion.
Key words: arteriosclerosis • restenosis • stents • genes • smooth muscle
This article has been cited by other articles:
 |
|
 |
|
  M. Zhang, H. Fang, J. Zhou, and B. P. Herring A Novel Role of Brg1 in the Regulation of SRF/MRTFA-dependent Smooth Muscle-specific Gene Expression J. Biol. Chem., August 31, 2007; 282(35): 25708 - 25716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.P.J.J. Bijnens, E. Lutgens, T. Ayoubi, J. Kuiper, A.J. Horrevoets, and M.J.A.P. Daemen Genome-Wide Expression Studies of Atherosclerosis: Critical Issues in Methodology, Analysis, Interpretation of Transcriptomics Data Arterioscler. Thromb. Vasc. Biol., June 1, 2006; 26(6): 1226 - 1235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. X.-F. Deng, J. M. Spin, A. Tsalenko, A. Vailaya, A. Ben-Dor, Z. Yakhini, P. Tsao, L. Bruhn, and T. Quertermous Molecular Signatures Determining Coronary Artery and Saphenous Vein Smooth Muscle Cell Phenotypes: Distinct Responses to Stimuli Arterioscler. Thromb. Vasc. Biol., May 1, 2006; 26(5): 1058 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lutgens, B. Faber, K. Schapira, C. T.A. Evelo, R. van Haaften, S. Heeneman, K. B.J.M. Cleutjens, A. P. Bijnens, L. Beckers, J. G. Porter, et al. Gene Profiling in Atherosclerosis Reveals a Key Role for Small Inducible Cytokines: Validation Using a Novel Monocyte Chemoattractant Protein Monoclonal Antibody Circulation, June 28, 2005; 111(25): 3443 - 3452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Pullmann Jr., M. Juhaszova, I. L. de Silanes, T. Kawai, K. Mazan-Mamczarz, M. K. Halushka, and M. Gorospe Enhanced Proliferation of Cultured Human Vascular Smooth Muscle Cells Linked to Increased Function of RNA-binding Protein HuR J. Biol. Chem., June 17, 2005; 280(24): 22819 - 22826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. N. Hahn, Z. J. Su, C. J. Drogemuller, A. Tsykin, S. R. Waterman, P. J. Brautigan, S. Yu, G. Kremmidiotis, A. Gardner, P. J. Solomon, et al. Expression profiling reveals functionally important genes and coordinately regulated signaling pathway genes during in vitro angiogenesis Physiol Genomics, June 16, 2005; 22(1): 57 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Herrmann New kits on the blot--can we microarray the future of atherosclerosis? Cardiovasc Res, November 1, 2003; 60(2): 220 - 222. [Full Text] [PDF]
|
|