on August 15, 2002
Arteriosclerosis, Thrombosis, and Vascular Biology. 2002
Published online before print August 15, 2002, doi: 10.1161/01.ATV.0000033829.14012.18
Published online before print August 15, 2002, doi: 10.1161/01.ATV.0000033829.14012.18
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Submitted on December 26, 2001
Accepted on August 4, 2002
Oxidized LDL in Carotid Plaques and Plasma Associates With Plaque Instability
Kyoko Nishi ;
From the Department of Neurosurgery (K.N., M.U., K.T.K., K.S., S.N.) and Pathology (H.H.), School of Medicine, The University of Tokushima, Tokushima, Japan, and Department of Molecular Pathology (H.I.), Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
* To whom correspondence should be addressed. E-mail: muno{at}clin.med.tokushima-u.ac.jp.
Objective—Oxidation of LDL plays a significant pathogenic role in atherosclerosis. In this study, we attempted to clarify the correlation between the morphology of human atherosclerotic plaques and the oxidized LDL (OxLDL) levels in plasma and carotid plaques.
Methods and Results—OxLDL levels (ng/µg apolipoprotein B) in plasma and carotid plaques from 44 patients undergoing carotid endarterectomy and OxLDL levels in 17 control plasma and 9 normal intima samples were determined by a sandwich ELISA by using specific antibodies against OxLDL (DLH3) and apolipoprotein B. The plaques were immunohistochemically classified as macrophage (M
)-rich and M-poor. In paired samples from individual patients, plaque OxLDL was nearly 70 times higher than plasma OxLDL (mean±SEM, 11.9±1.7 vs 0.18±0.01 ng/µg apoB, P<0.0001). The OxLDL level was significantly higher in M-rich- than M-poor plaques (19.6±2.8 vs 5.50±0.77ng/µg apoB, P<0.0001) and corresponded with DLH3 antigen positivity of the plaques. In patients with M-rich plaques, plasma OxLDL was significantly higher than in the controls (0.20±0.02 vs 0.13±0.01ng/µg apoB, P=0.02).
Conclusions—Our results suggest that LDL undergoes further oxidation in plaques, and that high plasma and plaque levels of OxLDL are correlated with the vulnerability to rupture of atherosclerotic lesions.
Key words: oxidized LDL • plaque • plasma • carotid endarterectomy • histopathology
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